{"id":"W2547216470","doi":"10.1101/pdb.prot090027","title":"The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein–Protein Interactions","year":2016,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Université Laval; PROTEO; Université de Montréal","funders":"","keywords":"Dihydrofolate reductase; Protein-fragment complementation assay; Complementation; Saccharomyces cerevisiae; Molecular biology; Biology; Biochemistry; Yeast; Chemistry; Enzyme; Gene; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001547384,0.0001942847,0.0002401184,0.0001379541,0.0003488234,0.00007943197,0.000306627,0.00009239075,0.0000457585],"category_scores_gemma":[0.0002894098,0.000134106,0.0002334616,0.0003941929,0.00006929952,0.0000121928,0.000164982,0.0001083654,0.000006346547],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000853532,"about_ca_system_score_gemma":0.0001565345,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003993222,"about_ca_topic_score_gemma":0.0002933391,"domain_scores_codex":[0.9979476,0.0003131765,0.0004715725,0.0004674286,0.0003519,0.0004482873],"domain_scores_gemma":[0.9986466,0.00005802278,0.0002837683,0.0005066142,0.00039784,0.000107136],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004828661,0.0001954326,0.001035339,0.00008109329,0.0004375092,2.849266e-7,0.00001184247,0.00003262567,0.9944768,0.002224083,0.00007397508,0.0009481827],"study_design_scores_gemma":[0.001084598,0.0004786834,0.001311911,0.0001368686,0.00006870251,1.153927e-7,0.00001638257,0.001041799,0.9352266,0.00007375149,0.06037961,0.0001810179],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.730801,0.00009262234,0.144871,0.001066155,0.00008548929,0.1225044,0.0003796437,0.00004683669,0.0001528132],"genre_scores_gemma":[0.8267359,0.00000464335,0.001925656,0.000009946559,0.0001288731,0.1695397,0.00001814575,0.00003212384,0.001604992],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1429454,"threshold_uncertainty_score":0.5468684,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02470814035812019,"score_gpt":0.3344359905067998,"score_spread":0.3097278501486797,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}