{"id":"W2547483025","doi":"10.1007/978-1-4939-6581-6_6","title":"Quantitative Live-Cell Fluorescence Microscopy During Phagocytosis","year":2016,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Phagocytosis and Immune Regulation","field":"Immunology and Microbiology","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"St. Michael's Hospital; Hospital for Sick Children; University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Phagosome; Phagocytosis; Cell biology; Confocal microscopy; Vacuole; Fluorescence microscope; Cytoplasm; Biology; Confocal; Effector; Intracellular; Macrophage; Phagolysosome; Green fluorescent protein; Chemistry; Fluorescence; Biochemistry; In vitro; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001143142,0.0006140015,0.0006505452,0.0005059899,0.0007632217,0.0009870708,0.001217748,0.00127776,0.002813349],"category_scores_gemma":[0.000643969,0.0005247691,0.0003431661,0.000499466,0.0007778002,0.0009720882,0.0006045105,0.002452483,0.00113362],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001309074,"about_ca_system_score_gemma":0.0007675056,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001113488,"about_ca_topic_score_gemma":0.001691451,"domain_scores_codex":[0.9990399,0.0001897972,0.00005104377,0.0002273868,0.0003120367,0.0001796996],"domain_scores_gemma":[0.9991737,0.0003795841,0.00008821006,0.000136137,0.0001704128,0.00005184806],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001047945,0.00002845346,0.0001696853,0.00009309906,0.000005590948,0.00003356498,0.00007377374,0.0002555191,0.9929606,0.001449718,0.0002712637,0.004553974],"study_design_scores_gemma":[0.00001586888,0.00004822805,0.001554542,0.0000133613,0.00000841005,0.0001231159,0.00003137451,0.009127957,0.9860052,0.0005605872,0.002496725,0.00001447859],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4439247,0.002568954,0.5376208,0.0008004121,0.0001948256,0.0002754434,0.001667219,0.002435807,0.01051196],"genre_scores_gemma":[0.6936332,0.001612182,0.2895962,0.000389217,0.0001012842,0.0008263986,0.001118938,0.0006756625,0.01204694],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002813349,"threshold_uncertainty_score":0.009498,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02180640101735327,"score_gpt":0.3707302857802498,"score_spread":0.3489238847628965,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}