{"id":"W2554164266","doi":"10.1016/j.pep.2016.11.004","title":"The expression of NTPDase1 and -2 of Leishmania infantum chagasi in bacterial and mammalian cells: Comparative expression, refolding and nucleotidase characterization","year":2016,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Adenosine and Purinergic Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Fonds de Recherche du Québec - Santé; Fundação de Amparo à Pesquisa do Estado de Minas Gerais; Canadian Institutes of Health Research; Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","keywords":"Biology; Leishmania infantum; Virulence; Microbiology; Leishmania; Enzyme; Nucleoside triphosphate; Biochemistry; 5'-nucleotidase; Cell biology; Nucleotide; Visceral leishmaniasis; Genetics; Leishmaniasis; Gene; Parasite hosting","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002111305,0.0001291995,0.0001591579,0.00005030206,0.0001154812,0.00002545261,0.00006773025,0.0001143867,0.000007836481],"category_scores_gemma":[0.00004701217,0.00007905721,0.00001403781,0.00004481904,0.0001511538,0.00003423813,0.0001081165,0.00004966232,1.449411e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003237809,"about_ca_system_score_gemma":0.00001308627,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009340522,"about_ca_topic_score_gemma":0.000001430303,"domain_scores_codex":[0.9990501,0.0001472951,0.0003092679,0.0002842376,0.00009303664,0.0001160596],"domain_scores_gemma":[0.9993923,0.00003583811,0.000272129,0.0001786143,0.00006054017,0.0000605907],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003046625,0.0000259452,0.001420979,0.00006120311,0.000002689667,2.758806e-7,0.0004968994,2.416371e-7,0.994516,0.00006976056,0.00006299825,0.003038323],"study_design_scores_gemma":[0.0007483316,0.00009851526,0.01134545,0.0004472136,0.000004479332,0.000002594102,0.0002149849,0.00002510791,0.9845965,0.0000743028,0.002335358,0.0001071634],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972059,0.001653805,0.0005256598,0.0001498347,0.00003043025,0.0003828035,0.00002590405,0.000004479599,0.00002120339],"genre_scores_gemma":[0.9981513,0.001129604,0.0004621547,0.000007726431,0.00005914867,0.00004679638,0.00003660178,0.00001013662,0.00009653644],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.009924469,"threshold_uncertainty_score":0.3223859,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01145904515650261,"score_gpt":0.2346686296220869,"score_spread":0.2232095844655843,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}