{"id":"W2556075107","doi":"10.1093/glycob/cww117","title":"Glycosyltransfer in mutants of putative catalytic residue Glu303 of the human ABO(H) A and B blood group glycosyltransferases GTA and GTB proceeds through a labile active site","year":2016,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta; University of Victoria","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Chemistry; Nucleophile; Oxocarbenium; Active site; Stereochemistry; Mutant; Glycosyltransferase; Residue (chemistry); Anomer; ABO blood group system; Hydrogen bond; Enzyme; Catalysis; Biochemistry; Biology; Genetics; Molecule","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002487109,0.0002465151,0.0003971359,0.0001074817,0.00008469466,0.000008452183,0.0002453187,0.0002830049,0.00002798307],"category_scores_gemma":[0.0002374174,0.0001558978,0.00008275914,0.0002721887,0.0009214107,0.00002117402,0.0001661478,0.0001528576,0.000001599713],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000176017,"about_ca_system_score_gemma":0.0001045287,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005222842,"about_ca_topic_score_gemma":0.001214822,"domain_scores_codex":[0.9982292,0.0002301465,0.0004423312,0.0005534044,0.0001595577,0.0003853361],"domain_scores_gemma":[0.9991645,0.0001187477,0.0001201881,0.0003577947,0.0001661676,0.00007257876],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006540695,0.0001707418,0.02751929,0.0001005773,0.0001142781,0.000005522661,0.0006435583,0.000001047204,0.9679715,0.001072674,0.0000159469,0.0017308],"study_design_scores_gemma":[0.003431755,0.001262366,0.06270146,0.000102363,0.0000487082,0.00007145426,0.0001647008,0.000002984585,0.9295226,0.001941097,0.0005336109,0.0002168612],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970633,0.0007781442,0.0001369692,0.0002648531,0.00003812031,0.0007361814,0.0004040617,0.000007725444,0.0005706401],"genre_scores_gemma":[0.9989974,0.0005329725,0.00009519143,0.0000602221,0.00003632886,0.00007044476,0.00006258251,0.0000242089,0.0001206217],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03844886,"threshold_uncertainty_score":0.6357329,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009070414901822922,"score_gpt":0.2583837084187893,"score_spread":0.2493132935169664,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}