{"id":"W2556587095","doi":"10.3791/54792","title":"Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to <em>S</em>-citalopram","year":2016,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canadian Institutes of Health Research; National Institute of Mental Health; Howard Hughes Medical Institute; National Institutes of Health; National Science Foundation","keywords":"Citalopram; Serotonin transporter; Serotonin; Transporter; Serotonin Plasma Membrane Transport Proteins; Chemistry; Serotonin Uptake Inhibitors; Serotonin reuptake inhibitor; Biochemistry; Pharmacology; Biology; Receptor; Fluoxetine; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003736661,0.0003305206,0.0003754602,0.0001443638,0.0002969308,0.0003322972,0.0004672545,0.0003294682,0.0008007674],"category_scores_gemma":[0.0003666948,0.0002470015,0.0002959151,0.0004679332,0.00022427,0.0001612709,0.0002487153,0.000712719,0.0006622699],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006697034,"about_ca_system_score_gemma":0.0003800512,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003799849,"about_ca_topic_score_gemma":0.006553772,"domain_scores_codex":[0.999778,0.00004966749,0.00003333548,0.00004185103,0.00005805616,0.00003905446],"domain_scores_gemma":[0.9999187,0.0000187295,0.00001441687,0.00001729368,0.00002012692,0.00001069232],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007833139,0.00003588999,0.0001930657,0.00005084217,0.00001872894,0.0001168924,0.00006524622,0.000480143,0.9963903,0.0002396723,0.0002834193,0.002047413],"study_design_scores_gemma":[0.00003287194,0.00006217247,0.0017187,0.000009443888,0.00002016463,0.0001786844,0.00002876415,0.002713321,0.9886835,0.00005924973,0.006481737,0.00001136354],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.962988,0.002899235,0.02746,0.0006273609,0.00009374777,0.0002596063,0.001804961,0.0002690729,0.003597867],"genre_scores_gemma":[0.9286605,0.004253814,0.05119459,0.0002265547,0.00003695784,0.000305034,0.006916501,0.0002374042,0.008168651],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003799849,"threshold_uncertainty_score":0.007555485,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01654827670063953,"score_gpt":0.3278059975650304,"score_spread":0.3112577208643909,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}