{"id":"W2558682509","doi":"10.1021/acschembio.6b00876","title":"Catalytic Promiscuity of <i>O</i>-GlcNAc Transferase Enables Unexpected Metabolic Engineering of Cytoplasmic Proteins with 2-Azido-2-deoxy-glucose","year":2016,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Canadian Institutes of Health Research; Institute of Genetics; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Nucleotide sugar; Transferase; Biochemistry; Glycosyltransferase; Uridine diphosphate; Enzyme; Cytoplasm; Chemistry; Glycobiology; Nucleotide; N-Acetylglucosamine; Uridine; Biology; Cell biology; Glycoprotein; Gene; RNA; Glycan","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001775786,0.0004628669,0.000246494,0.0001036212,0.0001134089,0.0005843705,0.0003502766,0.0002624347,0.0003684718],"category_scores_gemma":[0.000130162,0.0001547533,0.0003204916,0.0001801051,0.0003353076,0.0002596272,0.0003542195,0.0006153159,0.0002416891],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003491094,"about_ca_system_score_gemma":0.0002075377,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005162993,"about_ca_topic_score_gemma":0.0006882892,"domain_scores_codex":[0.9998709,0.00001995383,0.00001367295,0.00003286744,0.00003297167,0.00002951258],"domain_scores_gemma":[0.9999191,0.00001063289,0.00003049193,0.00001466504,0.000006219242,0.000018916],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000311021,0.000008428615,0.0001096624,0.00001594462,0.000003776076,0.00002945502,0.000006707686,0.00008768414,0.9989247,0.0001915691,0.00001734957,0.0005736609],"study_design_scores_gemma":[0.000004478215,0.00005920765,0.0006486826,0.000002532471,0.000009230507,0.0001214585,0.00001134911,0.0008933331,0.9957634,0.00007217968,0.002410231,0.00000373243],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9783368,0.0009240404,0.01785863,0.0002589711,0.00005240001,0.00003266661,0.0004122932,0.0002252331,0.00189907],"genre_scores_gemma":[0.9882934,0.0008569469,0.008833037,0.0000585808,0.000009406247,0.00002413027,0.0005274314,0.00006773706,0.0013293],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005843705,"threshold_uncertainty_score":0.002532959,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007167799265741292,"score_gpt":0.226854733798694,"score_spread":0.2196869345329527,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}