{"id":"W2559752217","doi":"10.1016/bs.mie.2016.11.002","title":"Activity Assays for Rhomboid Proteases","year":2016,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research","keywords":"Rhomboid; Proteases; Serine protease; Protease; Proteolysis; Subtilisin; Biochemistry; Chemistry; Serine; Catalytic triad; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","research_integrity"],"consensus_categories":[],"category_scores_codex":[0.006606366,0.0007741111,0.001357527,0.0007320841,0.0001390044,0.00002244921,0.001087192,0.002756881,0.0001407557],"category_scores_gemma":[0.00640401,0.0006276904,0.0003411674,0.0005067011,0.001098363,0.0000147742,0.0006356803,0.0007349679,0.00001918686],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001727803,"about_ca_system_score_gemma":0.000241896,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009378721,"about_ca_topic_score_gemma":0.0006664629,"domain_scores_codex":[0.988932,0.006282486,0.001073189,0.002001422,0.0001540183,0.00155691],"domain_scores_gemma":[0.9953735,0.002292393,0.0004759984,0.001505052,0.0001629601,0.000190049],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0011491,0.0004776243,0.002332503,0.0000333106,0.00007680956,0.00002934313,0.00002875885,0.00001368017,0.7795564,0.002646801,0.0003979015,0.2132578],"study_design_scores_gemma":[0.00364817,0.003678943,0.04650027,0.00005908007,0.00004020381,0.0002078619,0.00002951775,0.00002597702,0.8619937,0.02182165,0.06111199,0.0008825993],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6899849,0.0006686107,0.3022041,0.002098981,0.002341611,0.001659089,0.0001065938,0.00009086356,0.0008452085],"genre_scores_gemma":[0.275053,0.000383072,0.7213124,0.000811052,0.0004454668,0.001028372,0.00003169317,0.0001152774,0.0008196326],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.4191083,"threshold_uncertainty_score":0.9996175,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04365965110632703,"score_gpt":0.402527337981368,"score_spread":0.3588676868750409,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}