{"id":"W2560226640","doi":"10.1016/bs.mie.2016.10.006","title":"Biotin Switch Assays for Quantitation of Reversible Cysteine Oxidation","year":2016,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Redox biology and oxidative stress","field":"Biochemistry, Genetics and Molecular Biology","cited_by":27,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Cysteine; S-Nitrosylation; Chemistry; Biotin; Biotinylation; Biochemistry; Proteomics; Quantitative proteomics; Thiol; Glutathione; Combinatorial chemistry; Oxidative phosphorylation; Nitrosylation; Enzyme; Organic chemistry; Nitric oxide","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001504585,0.003143487,0.001721603,0.002140827,0.001323446,0.001024649,0.003026053,0.001660351,0.01156231],"category_scores_gemma":[0.001889704,0.001420179,0.001034262,0.001699512,0.0009572643,0.001243712,0.001665604,0.005476994,0.00460637],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001793999,"about_ca_system_score_gemma":0.001361433,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002209091,"about_ca_topic_score_gemma":0.0061633,"domain_scores_codex":[0.9972241,0.0005193265,0.0001850032,0.0006827649,0.0009797716,0.0004091016],"domain_scores_gemma":[0.9982446,0.0005749181,0.0001503449,0.0004074164,0.0003721137,0.0002506589],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001732435,0.00007679876,0.0001027827,0.0001324075,0.00002230062,0.00002381787,0.00005709203,0.00007380384,0.9927739,0.0009359234,0.0004390335,0.005188863],"study_design_scores_gemma":[0.0000175035,0.00007935463,0.0003418292,0.00001126115,0.00001614289,0.00005974228,0.00001115466,0.001421669,0.995654,0.0002609299,0.002114106,0.00001233997],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1601159,0.004120867,0.8031342,0.0004209169,0.0007522112,0.001351271,0.00392409,0.01062046,0.01556022],"genre_scores_gemma":[0.3967068,0.005053725,0.5222434,0.00044614,0.0001362706,0.003508627,0.01151152,0.001383667,0.05900988],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01156231,"threshold_uncertainty_score":0.03867978,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05014159424338419,"score_gpt":0.4297001062276921,"score_spread":0.3795585119843079,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}