{"id":"W2560250850","doi":"10.1007/978-1-4939-6424-6_18","title":"Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling","year":2016,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"Institute for Research in Immunology and Cancer; Université de Montréal","funders":"Canadian Cancer Society Research Institute; Canadian Institutes of Health Research; Health Canada; Canada Research Chairs; Cancer Research Institute","keywords":"Embryonic stem cell; Biology; Blastocyst; Cell biology; Stem cell; Homologous recombination; Homologous chromosome; Gene targeting; Embryo; Genetics; Gene; Embryogenesis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009816866,0.0007712238,0.0005284615,0.001628566,0.00053612,0.0005633214,0.0008417628,0.0005189832,0.002566829],"category_scores_gemma":[0.0004284363,0.0006368382,0.0006206024,0.0008067179,0.0003889122,0.0005107518,0.0004648644,0.00185377,0.002415117],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003339807,"about_ca_system_score_gemma":0.0004427373,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006248466,"about_ca_topic_score_gemma":0.001591948,"domain_scores_codex":[0.9994922,0.00008077522,0.00009430791,0.00006624737,0.0001919606,0.00007443527],"domain_scores_gemma":[0.9995769,0.00009432653,0.00006709803,0.0001007658,0.0000915586,0.00006941148],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001394125,0.00007066508,0.0002574439,0.00006376306,0.00001242267,0.00009872652,0.00004270997,0.00006893892,0.9947088,0.001033434,0.0003540788,0.003149584],"study_design_scores_gemma":[0.00008734146,0.0001786836,0.002228073,0.00003523395,0.00007060039,0.0003656261,0.00003254332,0.0006209802,0.9740691,0.0003488109,0.0219508,0.00001224959],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5058981,0.01757958,0.4262097,0.001837258,0.001026806,0.004118379,0.02173776,0.002059359,0.0195331],"genre_scores_gemma":[0.5598244,0.018254,0.3569734,0.00117123,0.0002615936,0.004571853,0.03157257,0.0009356111,0.02643533],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002566829,"threshold_uncertainty_score":0.008586824,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03011719929387805,"score_gpt":0.3719633204572415,"score_spread":0.3418461211633634,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}