{"id":"W2560739694","doi":"10.1016/j.jbiotec.2016.12.003","title":"Altering the central carbon metabolism of HEK293 cells: Impact on recombinant glycoprotein quality","year":2016,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada; Polytechnique Montréal","funders":"Fonds de recherche du Québec – Nature et technologies; Wallonie-Bruxelles International","keywords":"Recombinant DNA; Glycoprotein; Glycosylation; HEK 293 cells; Metabolism; Biochemistry; Cell culture; Biology; Chemistry; Cell biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004979963,0.0004890675,0.0004915255,0.0002293805,0.0002383187,0.001387366,0.0005208654,0.0006036671,0.002024347],"category_scores_gemma":[0.0006443734,0.000174552,0.0002960644,0.0003832414,0.0003239457,0.0004602142,0.0003565581,0.0008451311,0.0006754762],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005417603,"about_ca_system_score_gemma":0.0005566258,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001141631,"about_ca_topic_score_gemma":0.001038738,"domain_scores_codex":[0.9992895,0.000190604,0.0001112198,0.0001249937,0.0001933067,0.0000902471],"domain_scores_gemma":[0.9994912,0.0001917737,0.00009381848,0.00009988583,0.00006557423,0.00005777388],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004231461,0.00005392209,0.0001722155,0.0000228614,0.00000786879,0.00003208946,0.0000166334,0.00008630657,0.9977216,0.0001407605,0.00005305806,0.001269452],"study_design_scores_gemma":[0.0000195887,0.00009146626,0.0005335739,0.000002841303,0.00001612799,0.00006674822,0.000014398,0.0005242947,0.9973382,0.00004286953,0.001344461,0.000005338954],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9856974,0.001061416,0.008047205,0.0006087177,0.0001473565,0.00007695118,0.001727746,0.0002060316,0.00242715],"genre_scores_gemma":[0.986805,0.0005473971,0.007922446,0.0001719748,0.00002064027,0.00004976733,0.001977832,0.0002279381,0.002277125],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002024347,"threshold_uncertainty_score":0.006772161,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009161454300461629,"score_gpt":0.279527410199918,"score_spread":0.2703659558994564,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}