{"id":"W2561595298","doi":"10.1101/pdb.prot088724","title":"Synchronization and Arrest of the Budding Yeast Cell Cycle Using Chemical and Genetic Methods","year":2017,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Nocodazole; Cell cycle; Cell biology; Yeast; Biology; Cell synchronization; Population; Cell cycle checkpoint; Saccharomyces cerevisiae; Genetics; Cell; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001742798,0.00009165236,0.0001007326,0.00001545493,0.0001986678,0.00008450445,0.0002484996,0.00008476126,0.000002528824],"category_scores_gemma":[0.0001399748,0.00007568897,0.00002908975,0.00002816797,0.0001854752,0.000004257399,0.0005036413,0.00007781023,3.591532e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008986564,"about_ca_system_score_gemma":0.00006299627,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002236322,"about_ca_topic_score_gemma":0.000001992654,"domain_scores_codex":[0.9993226,0.00005694991,0.0001298318,0.0002305732,0.0001015348,0.000158495],"domain_scores_gemma":[0.9993157,0.000008437793,0.0001104324,0.0004408147,0.00006051458,0.00006407638],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001681577,0.00001802581,0.01611612,0.0001209009,0.000007657349,5.867651e-7,0.000006136001,0.00004912802,0.982811,0.00005231583,0.000002926554,0.0007984011],"study_design_scores_gemma":[0.0003423663,0.00004169643,0.02878653,0.00008271654,0.000008025609,0.000001180644,0.000002756466,0.004165949,0.9655955,0.00001293028,0.0008699382,0.00009043665],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9905445,0.0002236695,0.002859463,0.0000262638,0.00003348353,0.006207253,0.000004646437,0.000003392805,0.00009733195],"genre_scores_gemma":[0.9848235,0.00001972496,0.01364886,0.000009407974,0.0001004767,0.001354328,1.85477e-7,0.00001732949,0.00002617345],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01721551,"threshold_uncertainty_score":0.3086507,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02532689433807448,"score_gpt":0.3462286119690326,"score_spread":0.3209017176309581,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}