{"id":"W2561595298","doi":"10.1101/pdb.prot088724","title":"Synchronization and Arrest of the Budding Yeast Cell Cycle Using Chemical and Genetic Methods","year":2017,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Nocodazole; Cell cycle; Cell biology; Yeast; Biology; Cell synchronization; Population; Cell cycle checkpoint; Saccharomyces cerevisiae; Genetics; Cell; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001195052,0.0009052602,0.0008468711,0.001152586,0.00104332,0.0007966654,0.001509324,0.0005860075,0.003596671],"category_scores_gemma":[0.0007461015,0.0006117722,0.0009597546,0.0009984067,0.0008340855,0.0004455823,0.001642371,0.002315452,0.002911803],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006957793,"about_ca_system_score_gemma":0.001001982,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001449102,"about_ca_topic_score_gemma":0.00366217,"domain_scores_codex":[0.9976948,0.0003119615,0.0003773564,0.0004474978,0.0009928865,0.0001755302],"domain_scores_gemma":[0.9993976,0.00009710595,0.0001041519,0.0002313671,0.00009844908,0.00007131862],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002027356,0.00007252368,0.000247648,0.000285109,0.00001928557,0.0001038968,0.0001083524,0.0004616032,0.982002,0.002316875,0.001255556,0.01292436],"study_design_scores_gemma":[0.00008355095,0.0002310623,0.001297702,0.00005049429,0.00002908156,0.0003169599,0.00004978499,0.002076091,0.9394703,0.0005401204,0.05580191,0.00005288447],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2375417,0.01176697,0.6916194,0.001377777,0.002365579,0.008304007,0.00934913,0.004608478,0.03306688],"genre_scores_gemma":[0.3126988,0.01224685,0.6234224,0.0008410906,0.0003297729,0.009718177,0.008923345,0.001738605,0.03008095],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003596671,"threshold_uncertainty_score":0.01203203,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02532689433807448,"score_gpt":0.3462286119690326,"score_spread":0.3209017176309581,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}