{"id":"W2565032632","doi":"10.1016/s1525-0016(16)33101-x","title":"292. Towards Large-Scale Manufacturing of Adeno-Associated Virus by Transient Transfection of HEK293 Suspension Cells in a Stirred Tank Bioreactor Using Serum-Free Medium","year":2016,"lang":"en","type":"article","venue":"Molecular Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; National Research Council Canada","funders":"","keywords":"Polyethylenimine; Transfection; Plasmid; HEK 293 cells; Bioreactor; Chromatography; Viral vector; Adeno-associated virus; Cell culture; Gene delivery; Baby hamster kidney cell; Chemistry; Molecular biology; Biology; Virus; Virology; Gene; Vector (molecular biology); Biochemistry; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000565468,0.0002872789,0.0003940254,0.0001729779,0.00004270059,0.00001024076,0.0003823843,0.0003151746,0.00009987718],"category_scores_gemma":[0.00002716106,0.0002350853,0.0002338544,0.0002061859,0.000119768,0.00001117991,0.00004900079,0.0001379069,0.000001625639],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008893646,"about_ca_system_score_gemma":0.0001266882,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000230134,"about_ca_topic_score_gemma":0.0004703989,"domain_scores_codex":[0.9976911,0.0003483126,0.0004737377,0.0004936763,0.0004933917,0.0004998154],"domain_scores_gemma":[0.999032,0.00002154883,0.0001541714,0.0005551815,0.0001271262,0.000109956],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002150919,0.0005492205,0.0001731176,0.0000207903,0.0001607615,0.000008350386,0.0002228154,0.0000726467,0.9904923,4.72636e-7,0.00009958853,0.006049052],"study_design_scores_gemma":[0.006127278,0.001734365,0.001402673,0.00007804855,0.00001813368,0.000002674946,0.00004382624,0.00007007905,0.9896533,0.00003869773,0.0005676507,0.0002632849],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9935384,0.001442386,0.003708705,0.0002105345,0.0001048645,0.0005201752,0.0004292343,0.00001580786,0.00002987009],"genre_scores_gemma":[0.998207,0.001336251,0.0001309642,0.0000879656,0.00002787143,0.00001659718,0.00005226151,0.00007037874,0.00007074125],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005785767,"threshold_uncertainty_score":0.9586501,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01355278861287959,"score_gpt":0.2656788913549235,"score_spread":0.2521261027420439,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}