{"id":"W2565032632","doi":"10.1016/s1525-0016(16)33101-x","title":"292. Towards Large-Scale Manufacturing of Adeno-Associated Virus by Transient Transfection of HEK293 Suspension Cells in a Stirred Tank Bioreactor Using Serum-Free Medium","year":2016,"lang":"en","type":"article","venue":"Molecular Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; National Research Council Canada","funders":"","keywords":"Polyethylenimine; Transfection; Plasmid; HEK 293 cells; Bioreactor; Chromatography; Viral vector; Adeno-associated virus; Cell culture; Gene delivery; Baby hamster kidney cell; Chemistry; Molecular biology; Biology; Virus; Virology; Gene; Vector (molecular biology); Biochemistry; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006871893,0.0004924069,0.0004605532,0.0003502611,0.0003163289,0.0007109563,0.0005443383,0.0005841478,0.002575003],"category_scores_gemma":[0.0003385973,0.0003741022,0.000687325,0.0003360504,0.000279659,0.0002767493,0.0004094293,0.0008577813,0.006402581],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003678865,"about_ca_system_score_gemma":0.0003966164,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006374172,"about_ca_topic_score_gemma":0.0008216039,"domain_scores_codex":[0.9994181,0.00008756897,0.0000660386,0.0001281399,0.0002710706,0.00002914186],"domain_scores_gemma":[0.9998158,0.00003925264,0.0000313737,0.00003334068,0.00005893519,0.0000215049],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002305881,0.00003156121,0.00007240476,0.00006502948,0.000003915856,0.00006666475,0.00001969723,0.0001195462,0.9955858,0.000270565,0.0002567915,0.003484888],"study_design_scores_gemma":[0.00001497387,0.0001546553,0.0006286507,0.00001397353,0.00001478686,0.0003974413,0.00001221877,0.001949619,0.9800686,0.0001493456,0.01658143,0.00001426026],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.262642,0.003480936,0.6963341,0.0005762149,0.0005589624,0.002197382,0.006060122,0.003453592,0.02469672],"genre_scores_gemma":[0.3931182,0.004850365,0.5457234,0.0002962752,0.0001055799,0.00193441,0.01833966,0.0005299614,0.03510217],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002575003,"threshold_uncertainty_score":0.008614242,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01355278861287959,"score_gpt":0.2656788913549235,"score_spread":0.2521261027420439,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}