{"id":"W2565874618","doi":"10.2144/000114482","title":"High-Yield Recombinant Expression and Purification of Marginally Soluble, Short Elastin-Like Polypeptides","year":2016,"lang":"en","type":"article","venue":"BioTechniques","topic":"Connective tissue disorders research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Institute for Nanotechnology; University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Innovates; King Saud University","keywords":"Recombinant DNA; Elastin; Yield (engineering); Chemistry; Biochemistry; Molecular biology; Biology; Genetics; Gene; Materials science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002033042,0.0001063004,0.000118138,0.00006955133,0.00004208411,0.000009711864,0.0001450793,0.000178432,0.00003031968],"category_scores_gemma":[0.0001553405,0.00007689217,0.00003507936,0.00005543753,0.0001235868,0.000006764101,0.0001427546,0.00005140156,0.000002003191],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001067199,"about_ca_system_score_gemma":0.00003316646,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006732238,"about_ca_topic_score_gemma":0.0000235918,"domain_scores_codex":[0.9992222,0.00004720291,0.000158936,0.000294663,0.0001145191,0.0001624532],"domain_scores_gemma":[0.9994653,0.00002940308,0.0000508648,0.0002993424,0.0001075326,0.00004748847],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009916999,0.0000419966,0.002082361,0.00001807323,0.000009482863,6.634706e-7,0.000008020243,3.373277e-8,0.9608741,0.0002049339,0.002861029,0.03380013],"study_design_scores_gemma":[0.0001285751,0.0004076315,0.006511583,0.0000984073,0.00000394142,0.000004013862,0.00001419601,0.000001237861,0.981613,0.0003150982,0.01078832,0.0001139487],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9743354,0.0009900541,0.02314187,0.0006985671,0.00007712599,0.0003487077,0.00001164719,0.00004484811,0.0003517562],"genre_scores_gemma":[0.9920531,0.001756307,0.005027881,0.00001850018,0.00003364416,0.00004347141,0.00001437757,0.00001669157,0.001036053],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03368618,"threshold_uncertainty_score":0.3135572,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01959527900151851,"score_gpt":0.2777969929953927,"score_spread":0.2582017139938742,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}