{"id":"W2565874618","doi":"10.2144/000114482","title":"High-Yield Recombinant Expression and Purification of Marginally Soluble, Short Elastin-Like Polypeptides","year":2016,"lang":"en","type":"article","venue":"BioTechniques","topic":"Connective tissue disorders research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Institute for Nanotechnology; University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Innovates; King Saud University","keywords":"Recombinant DNA; Elastin; Yield (engineering); Chemistry; Biochemistry; Molecular biology; Biology; Genetics; Gene; Materials science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005052123,0.0005843956,0.0006094109,0.0003336527,0.0003455983,0.0005763587,0.0003906375,0.0004040678,0.001642698],"category_scores_gemma":[0.0003512179,0.0002816818,0.0003379348,0.0005582873,0.0002098105,0.0003699122,0.0004787036,0.0009585612,0.002240584],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002513248,"about_ca_system_score_gemma":0.0002779387,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002104907,"about_ca_topic_score_gemma":0.0004462088,"domain_scores_codex":[0.9996403,0.00005805891,0.00004876784,0.00006389891,0.0001331446,0.00005585803],"domain_scores_gemma":[0.9998202,0.00004286967,0.0000319855,0.00003614215,0.00004086773,0.00002803384],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001743733,0.00001848706,0.00005194098,0.00007229545,0.000003085592,0.00006584252,0.0000284389,0.00005798166,0.9974319,0.0001402567,0.0001113713,0.002000841],"study_design_scores_gemma":[0.00001140842,0.00009147833,0.0009703662,0.00001486789,0.00001415802,0.0003757109,0.00003087905,0.0008179345,0.9802446,0.0001373864,0.01728087,0.00001037201],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6998275,0.004696828,0.2753506,0.0004247761,0.0002958682,0.001258739,0.007053061,0.001590232,0.009502465],"genre_scores_gemma":[0.7008212,0.006363844,0.2319575,0.0001854933,0.0001000804,0.001272646,0.02167031,0.0006473885,0.0369815],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001642698,"threshold_uncertainty_score":0.005495369,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01959527900151851,"score_gpt":0.2777969929953927,"score_spread":0.2582017139938742,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}