{"id":"W2568142053","doi":"10.1021/acschembio.6b00971","title":"Fluorescent Branched RNAs for High-Throughput Analysis of Dbr1 Enzyme Kinetics and Inhibition","year":2017,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Division of Biological Infrastructure; Natural Sciences and Engineering Research Council of Canada; Judith and Jean Pape Adams Charitable Foundation; Japan Society for the Promotion of Science; Welch Foundation; U.S. Department of Veterans Affairs","keywords":"Phosphodiester bond; Phosphoramidate; Enzyme; Moiety; High-throughput screening; Biochemistry; Enzyme kinetics; Fluorescence; RNA; Chemistry; Kinetics; Oligonucleotide; Biology; DNA; Stereochemistry; Active site; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006312759,0.0006978588,0.0006110988,0.0006142954,0.000208183,0.0004640155,0.0005958669,0.0006040331,0.001660282],"category_scores_gemma":[0.0005991983,0.0003092778,0.0003616968,0.0005114794,0.0002173098,0.0004135236,0.0002461866,0.001326083,0.0007068743],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000542485,"about_ca_system_score_gemma":0.0002247611,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005597645,"about_ca_topic_score_gemma":0.001039235,"domain_scores_codex":[0.9994161,0.0001102213,0.00003429031,0.0001076153,0.0002715549,0.00006023052],"domain_scores_gemma":[0.9994532,0.0002752267,0.0000751831,0.00005525339,0.00008791077,0.00005311164],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004315957,0.00002927528,0.00005384198,0.00003691813,0.000005209904,0.00001239929,0.0000108738,0.0003341141,0.9972262,0.00008570054,0.00005289065,0.002109464],"study_design_scores_gemma":[0.000007307394,0.00009437907,0.000329585,0.000002698896,0.0000049926,0.00003014118,0.000004380013,0.003459891,0.9953741,0.00005550921,0.0006311825,0.000005804733],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6513562,0.00530707,0.330441,0.0003283705,0.0001300446,0.0003329313,0.004172089,0.002450792,0.005481421],"genre_scores_gemma":[0.726785,0.004355649,0.2524153,0.0002325698,0.00006866396,0.0008553853,0.004815332,0.0003811141,0.01009095],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001660282,"threshold_uncertainty_score":0.005554199,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01590876978574593,"score_gpt":0.300700367955456,"score_spread":0.2847915981697101,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}