{"id":"W2568142053","doi":"10.1021/acschembio.6b00971","title":"Fluorescent Branched RNAs for High-Throughput Analysis of Dbr1 Enzyme Kinetics and Inhibition","year":2017,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Division of Biological Infrastructure; Natural Sciences and Engineering Research Council of Canada; Judith and Jean Pape Adams Charitable Foundation; Japan Society for the Promotion of Science; Welch Foundation; U.S. Department of Veterans Affairs","keywords":"Phosphodiester bond; Phosphoramidate; Enzyme; Moiety; High-throughput screening; Biochemistry; Enzyme kinetics; Fluorescence; RNA; Chemistry; Kinetics; Oligonucleotide; Biology; DNA; Stereochemistry; Active site; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001311155,0.0001104235,0.0002677611,0.00005053528,0.00007346639,0.00001968105,0.0001806588,0.0002324816,0.000006960049],"category_scores_gemma":[0.0004628354,0.00009579107,0.00009534745,0.00005052597,0.0003156526,0.000003678769,0.0002047413,0.0000653526,7.248537e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008963757,"about_ca_system_score_gemma":0.00001688625,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006322668,"about_ca_topic_score_gemma":0.00002667384,"domain_scores_codex":[0.9991401,0.00002453485,0.0002062116,0.0003239381,0.00005580443,0.0002494204],"domain_scores_gemma":[0.9992269,0.00003941249,0.0001301921,0.0004173475,0.0001058253,0.00008028313],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001526075,0.00004710923,0.00277605,0.00001875426,0.0002626053,5.599912e-7,0.000007391024,0.00000163494,0.9925188,0.0005967544,0.0002466051,0.003371161],"study_design_scores_gemma":[0.0007506985,0.0002670106,0.003571636,0.000005537559,0.0001131519,0.00000137093,0.000005792248,0.0001085261,0.9935464,0.0004755461,0.001047741,0.0001065886],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9963375,0.0002926967,0.002600916,0.0004477292,0.00003695845,0.0001417969,0.00008658403,0.000004372454,0.00005149152],"genre_scores_gemma":[0.9977368,0.0002426142,0.001104845,0.000114236,0.0001310657,0.00001966402,0.0005776226,0.000009247483,0.00006391213],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003264573,"threshold_uncertainty_score":0.3906247,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01590876978574593,"score_gpt":0.300700367955456,"score_spread":0.2847915981697101,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}