{"id":"W2574401607","doi":"10.1038/ncomms14109","title":"Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification","year":2017,"lang":"en","type":"article","venue":"Nature Communications","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":146,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Institute for Research in Immunology and Cancer; Université du Québec à Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Agence Nationale de la Recherche; Fonds de Recherche du Québec - Santé; Genome Canada; Styrelsen för Internationellt Utvecklingssamarbete","keywords":"SUMO protein; Ubiquitin; Proteasome; MG132; Crosstalk; HEK 293 cells; Deubiquitinating enzyme; Immunoprecipitation; Cell biology; RNF4; Chemistry; Ubiquitin ligase; Biology; Biochemistry; Proteasome inhibitor; Gene; Transcription factor; Zinc finger","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002781519,0.000306145,0.0003114503,0.0003101669,0.0002190516,0.0005105542,0.0002400338,0.0002691492,0.001160291],"category_scores_gemma":[0.0001633757,0.000230244,0.0002331537,0.0003508523,0.0001767606,0.0002400111,0.0002411372,0.000395677,0.0009321216],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002441858,"about_ca_system_score_gemma":0.0002355779,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003066398,"about_ca_topic_score_gemma":0.0008426299,"domain_scores_codex":[0.9997516,0.00003418716,0.00002278883,0.00008391042,0.00007099746,0.00003652852],"domain_scores_gemma":[0.9998984,0.00002722723,0.00002041849,0.00002670135,0.00001390437,0.00001324174],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005483517,0.00001218017,0.0004085458,0.00002690086,0.00001080978,0.00006217515,0.00001383022,0.00003917877,0.9968478,0.00008549036,0.00003153779,0.00240669],"study_design_scores_gemma":[0.000007900141,0.00006645157,0.003037479,0.000003021554,0.00002128444,0.0004735058,0.00001888639,0.0005102261,0.9920444,0.0001254442,0.003687904,0.000003421973],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9165183,0.006573489,0.07138319,0.000259091,0.0000657998,0.0001293403,0.001287655,0.0002827439,0.00350036],"genre_scores_gemma":[0.9450858,0.005034302,0.04440995,0.0001588636,0.00003316829,0.00009597324,0.002018406,0.0000505755,0.003113091],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001160291,"threshold_uncertainty_score":0.003881574,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03747269746830289,"score_gpt":0.33574006241813,"score_spread":0.2982673649498271,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}