{"id":"W2580518546","doi":"10.1021/acs.biomac.6b01790","title":"Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products","year":2017,"lang":"en","type":"article","venue":"Biomacromolecules","topic":"Polysaccharides and Plant Cell Walls","field":"Agricultural and Biological Sciences","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Polysaccharide; Cellulose; Fluorophore; Fluorescence spectroscopy; Chromatography; Oxidizing agent; Oligosaccharide; Lytic cycle; Fluorescence; Monooxygenase; Biochemistry; Enzyme; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002445879,0.0001947526,0.000311255,0.00002567324,0.000306427,0.0000751404,0.0004928784,0.0001302964,0.00003432985],"category_scores_gemma":[0.0001509377,0.00009323184,0.0001176026,0.0001543147,0.0001560532,0.0001532716,0.00008803628,0.00008350618,0.000008306084],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001937917,"about_ca_system_score_gemma":0.00002799214,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.005169716,"about_ca_topic_score_gemma":0.0002462997,"domain_scores_codex":[0.9987487,0.00006231081,0.0002970621,0.000360724,0.0002222849,0.0003089637],"domain_scores_gemma":[0.9989476,0.00008634673,0.0005390829,0.0002210841,0.0001134723,0.00009237126],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001833581,0.0001610851,0.000443826,0.00004322557,0.00002305712,0.000006287487,0.00001528512,7.573773e-7,0.9824678,0.000004874747,0.00006783677,0.0165826],"study_design_scores_gemma":[0.0003085799,0.0001654451,0.006669667,0.0000605594,0.00002550933,0.000004691331,0.00001354186,0.0002546492,0.9920685,0.00002575368,0.0002121138,0.0001909882],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9986222,0.0003315168,0.00002231296,0.0002986473,0.0001393417,0.0002354758,0.000278241,0.00003430959,0.00003790195],"genre_scores_gemma":[0.9994382,0.00007632349,0.0003314608,0.00001547665,0.00004813542,0.000006979504,0.00002523693,0.000002435266,0.0000558025],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01639161,"threshold_uncertainty_score":0.7815098,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01631894020705029,"score_gpt":0.2155599260401134,"score_spread":0.1992409858330631,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}