{"id":"W2580860321","doi":"10.1139/cjm-2016-0528","title":"Expression, purification, and renaturation of a recombinant peptide-based HIV vaccine in <i>Escherichia coli</i>","year":2017,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"HIV Research and Treatment","field":"Immunology and Microbiology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Major Science and Technology Projects of China","keywords":"Epitope; Recombinant DNA; Escherichia coli; Immunogenicity; Inclusion bodies; Biology; Affinity chromatography; Antigen; Plasmid; Molecular biology; Population; Virology; Biochemistry; DNA; Gene; Enzyme","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004745726,0.0006188726,0.0006025488,0.0002776344,0.0002750866,0.000426429,0.000373518,0.0003213039,0.0005703706],"category_scores_gemma":[0.0003729156,0.0003409057,0.0004530374,0.0005512409,0.0001691707,0.0002986979,0.0003441464,0.00094513,0.0007349545],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003778345,"about_ca_system_score_gemma":0.0003770999,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001011855,"about_ca_topic_score_gemma":0.001181637,"domain_scores_codex":[0.9996766,0.0000924987,0.00004009904,0.0000516228,0.00006922192,0.00007002854],"domain_scores_gemma":[0.9998816,0.00002210578,0.00002253537,0.0000210163,0.00003320474,0.00001950073],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006290461,0.00004574183,0.0001676217,0.00005900821,0.000006304632,0.00006336994,0.00003189734,0.0004175697,0.9971442,0.0001822015,0.00006566477,0.001753582],"study_design_scores_gemma":[0.000009774472,0.000110936,0.0007184774,0.000007747406,0.0000117704,0.0001400615,0.00002079539,0.002818847,0.9924335,0.000056903,0.003662893,0.000008274139],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9199921,0.001827038,0.07323978,0.0002419417,0.0001095286,0.0004959254,0.001260163,0.0003104206,0.002522955],"genre_scores_gemma":[0.8075673,0.002923192,0.1761491,0.0001536459,0.00003604696,0.0003368626,0.004861652,0.0002407733,0.007731402],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001011855,"threshold_uncertainty_score":0.002741396,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01183146583633549,"score_gpt":0.2348980079660887,"score_spread":0.2230665421297532,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}