{"id":"W2581180556","doi":"10.1016/j.biochi.2017.01.014","title":"Production in Pichia pastoris, antifungal activity and crystal structure of a class I chitinase from cowpea (Vigna unguiculata): Insights into sugar binding mode and hydrolytic action","year":2017,"lang":"en","type":"article","venue":"Biochimie","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University","funders":"Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","keywords":"Chitinase; Pichia pastoris; Chitin; Biochemistry; Molecular mass; Affinity chromatography; Chemistry; Hydrolysis; Recombinant DNA; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002243798,0.0005735321,0.0005113493,0.0002704026,0.0002242566,0.0005532433,0.0005755424,0.0002956001,0.0007236718],"category_scores_gemma":[0.0001842694,0.0003039965,0.0003417642,0.0008047347,0.0001429186,0.0002460862,0.0002415428,0.0008537096,0.0006066039],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006649538,"about_ca_system_score_gemma":0.0002731773,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003496432,"about_ca_topic_score_gemma":0.003987374,"domain_scores_codex":[0.9998651,0.00001826826,0.00001445935,0.00002864437,0.00005237801,0.00002124677],"domain_scores_gemma":[0.9998924,0.0000237181,0.00002078142,0.00002371019,0.00001272237,0.00002675624],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001278922,0.00005431566,0.0002218656,0.0000658546,0.00002173023,0.00007190964,0.00003471531,0.0003874702,0.9968046,0.0001923622,0.0001484774,0.001868822],"study_design_scores_gemma":[0.00004051533,0.0001015575,0.002959672,0.000007918619,0.00004488061,0.0001710253,0.00002985044,0.001966558,0.9895422,0.00007597973,0.005049728,0.00001011167],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9769089,0.002065926,0.009712579,0.0004254008,0.0001072574,0.00009786897,0.006860019,0.0004152886,0.003406815],"genre_scores_gemma":[0.9582134,0.00181302,0.01277216,0.00008164983,0.00002520258,0.0001093377,0.01891953,0.0001374453,0.007928174],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003496432,"threshold_uncertainty_score":0.006952167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01186080566744028,"score_gpt":0.2630697981582412,"score_spread":0.2512089924908009,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}