{"id":"W2581180556","doi":"10.1016/j.biochi.2017.01.014","title":"Production in Pichia pastoris, antifungal activity and crystal structure of a class I chitinase from cowpea (Vigna unguiculata): Insights into sugar binding mode and hydrolytic action","year":2017,"lang":"en","type":"article","venue":"Biochimie","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University","funders":"Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","keywords":"Chitinase; Pichia pastoris; Chitin; Biochemistry; Molecular mass; Affinity chromatography; Chemistry; Hydrolysis; Recombinant DNA; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00004001864,0.0002070734,0.0002392417,0.00006351186,0.0003099909,0.00004646808,0.0001135085,0.0001567771,0.000003213119],"category_scores_gemma":[0.0001404082,0.0001897701,0.00004529375,0.00004217818,0.0002155706,0.00002055874,0.0002018196,0.0001243311,2.098083e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001859488,"about_ca_system_score_gemma":0.00003673565,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009905717,"about_ca_topic_score_gemma":0.005564839,"domain_scores_codex":[0.9989709,0.00004945388,0.0001775871,0.0005025358,0.0001207532,0.0001787215],"domain_scores_gemma":[0.9992646,0.00001324069,0.0002224376,0.0003969244,0.00003325976,0.00006956633],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002371814,0.00006135082,0.03289025,0.00006017792,0.00005918458,0.000003822489,0.000148557,0.00001540753,0.9646008,0.00001294046,0.00006551692,0.001844827],"study_design_scores_gemma":[0.0006128421,0.0001452406,0.3044624,0.00005731289,0.00003366972,0.000008406379,0.0001907124,0.0001305097,0.6939223,0.000107952,0.0001459417,0.0001827108],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980528,0.001152194,0.00001581074,0.0001101158,0.0003010493,0.0002005502,0.0001022536,0.00000847817,0.00005678727],"genre_scores_gemma":[0.998663,0.0006615419,0.0001234284,0.00001289959,0.000399375,0.000005215594,0.00009969925,0.00001830529,0.00001653342],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2715721,"threshold_uncertainty_score":0.77386,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01186080566744028,"score_gpt":0.2630697981582412,"score_spread":0.2512089924908009,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}