{"id":"W2583045994","doi":"10.2144/000114501","title":"Native protein denaturation using urea","year":2017,"lang":"en","type":"article","venue":"BioTechniques","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Carleton University","funders":"","keywords":"Denaturation (fissile materials); Cooperativity; Kinetics; Chemistry; Urea; Protein stability; Protein folding; Biophysics; Biochemistry; Biology; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001357919,0.002483172,0.001445421,0.001904886,0.001635135,0.0006399979,0.001890847,0.001003423,0.01946753],"category_scores_gemma":[0.001637378,0.001099329,0.000913744,0.001372164,0.000561089,0.001055272,0.0009011097,0.002838605,0.01417224],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006377131,"about_ca_system_score_gemma":0.001478032,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001066453,"about_ca_topic_score_gemma":0.002429314,"domain_scores_codex":[0.9982856,0.0003609502,0.0002445882,0.0004507285,0.0004409807,0.0002171781],"domain_scores_gemma":[0.9990786,0.0001533982,0.000101924,0.0002622554,0.0003298111,0.00007391837],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002261147,0.0001267167,0.0002873272,0.001663903,0.00007191179,0.0003682098,0.0002145851,0.0004460608,0.9709904,0.002403968,0.007844009,0.01535692],"study_design_scores_gemma":[0.00009919655,0.000552803,0.001688011,0.0003334825,0.0001018008,0.0009831958,0.0000727517,0.002573142,0.7878754,0.002049069,0.2035097,0.0001614196],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07688182,0.01074657,0.8363921,0.001047295,0.001657534,0.01663755,0.01940621,0.009674488,0.02755644],"genre_scores_gemma":[0.1438591,0.02214074,0.6255279,0.001650468,0.0003495047,0.05377445,0.06950659,0.002453888,0.08073732],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01946753,"threshold_uncertainty_score":0.06512541,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01524681447586981,"score_gpt":0.303078169329271,"score_spread":0.2878313548534012,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}