{"id":"W2586544602","doi":"10.1074/jbc.m116.757898","title":"Negative Stain Single-particle EM of the Maltose Transporter in Nanodiscs Reveals Asymmetric Closure of MalK2 and Catalytic Roles of ATP, MalE, and Maltose","year":2017,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia; McGill University","funders":"Canadian Institutes of Health Research; Fonds de Recherche du Québec - Santé; Compute Canada; Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Periplasmic space; ATP-binding cassette transporter; Maltose; ATP hydrolysis; ATPase; Biochemistry; Maltose-binding protein; Conformational change; Escherichia coli; Transporter; Biology; Biophysics; Chemistry; Enzyme; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000866309,0.0001831464,0.000173427,0.00008982603,0.0001412623,0.0002641573,0.0002919504,0.0002357576,0.0004985511],"category_scores_gemma":[0.0001163592,0.00009917581,0.0001522206,0.00009968477,0.0002210962,0.0002895385,0.0001440762,0.0003812777,0.0001645535],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004066186,"about_ca_system_score_gemma":0.00009120262,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009301898,"about_ca_topic_score_gemma":0.0008186818,"domain_scores_codex":[0.9999499,0.000005106208,0.000006329244,0.00001315635,0.00001489256,0.00001056933],"domain_scores_gemma":[0.999923,0.00002004902,0.00001661804,0.00001156237,0.00001369135,0.00001516474],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007475303,0.00001786351,0.0003454318,0.00002743378,0.000005157339,0.00009170531,0.00003471032,0.0002906201,0.9983554,0.0001262984,0.00003230791,0.0005982487],"study_design_scores_gemma":[0.000005712608,0.00005370141,0.0060357,0.000007377296,0.000008978439,0.0001926163,0.00008700754,0.004978097,0.9878117,0.0000676032,0.0007438239,0.000007686597],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9961883,0.0001832187,0.002885603,0.00004049165,0.00001069704,0.000003824202,0.0001666988,0.00002552932,0.0004955377],"genre_scores_gemma":[0.9960759,0.0002243779,0.002519616,0.00003580088,0.000002336981,0.000009587626,0.0003768043,0.00002621032,0.000729243],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009301898,"threshold_uncertainty_score":0.002950251,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02108198387630074,"score_gpt":0.2510722309312017,"score_spread":0.229990247054901,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}