{"id":"W2587354015","doi":"10.21769/bioprotoc.2082","title":"In vitro Ubiquitin Dimer Formation Assay","year":2017,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Ubiquitin; Ubiquitin-conjugating enzyme; Ubiquitin ligase; Lysine; Ubiquitin-Protein Ligases; Dimer; Enzyme; Deubiquitinating enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004236306,0.0001540744,0.0001253501,0.00004209371,0.000198923,0.00009946727,0.0003679181,0.0001907425,0.0001181283],"category_scores_gemma":[0.000149105,0.000139936,0.00007407353,0.00002795293,0.00007246216,0.00002107337,0.0001912309,0.0001036709,0.0001147634],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001876494,"about_ca_system_score_gemma":0.00004997618,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004027657,"about_ca_topic_score_gemma":0.00002979362,"domain_scores_codex":[0.9990108,0.00008279392,0.0002345604,0.0002688543,0.0001268631,0.0002761114],"domain_scores_gemma":[0.9990488,0.000006144902,0.0001858089,0.0006394533,0.00004868066,0.00007107868],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003264054,0.0000602151,0.0004958729,0.00006362896,0.000006628687,0.000005255518,0.00003108825,5.472692e-7,0.9875331,0.00009683907,0.003666323,0.007714109],"study_design_scores_gemma":[0.00105106,0.00008826849,0.0008511034,0.00001452464,0.000001553626,0.000005336119,0.000008629102,0.00003960415,0.6770498,0.0001294376,0.3206248,0.0001358975],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.04774365,0.00001044182,0.001090204,0.001082215,0.0002384229,0.8868582,0.00007134679,0.00005143272,0.06285405],"genre_scores_gemma":[0.2356721,6.170569e-7,0.0006398471,0.0003319532,0.000344551,0.7618044,0.0000368903,0.00001999588,0.00114972],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.3169585,"threshold_uncertainty_score":0.5706425,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02645826773201448,"score_gpt":0.3228470762924824,"score_spread":0.296388808560468,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}