{"id":"W2589100284","doi":"10.1039/c7ob00259a","title":"Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS","year":2017,"lang":"en","type":"article","venue":"Organic & Biomolecular Chemistry","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Glycosyltransferase; Dehydratase; Chemistry; Biochemistry; Biosynthesis; Gene; Substrate (aquarium); Natural product; Substrate specificity; Nucleotide sugar; Isolation (microbiology); Enzyme; Biology; Bioinformatics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008289816,0.0002978497,0.0002096549,0.0001441144,0.0001048113,0.0002698275,0.0001488315,0.000179771,0.0004073286],"category_scores_gemma":[0.0001174375,0.0001007517,0.0001775183,0.0001830116,0.0001456226,0.0001497987,0.0001900357,0.0004122581,0.0002262203],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001919412,"about_ca_system_score_gemma":0.0001990146,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005710794,"about_ca_topic_score_gemma":0.0007366075,"domain_scores_codex":[0.9999349,0.00001039008,0.000008758322,0.0000149188,0.00001621373,0.00001485261],"domain_scores_gemma":[0.9999022,0.00002127116,0.00001970117,0.0000167255,0.000009592841,0.00003052345],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001652408,0.000003642312,0.00008633496,0.000004652612,6.780283e-7,0.00002002065,0.000003100883,0.00001293762,0.9995406,0.00001049683,0.000002641598,0.0002983197],"study_design_scores_gemma":[0.000002185142,0.00002697594,0.001260998,9.134827e-7,0.000002605585,0.00009185688,0.00000968217,0.0001796717,0.9978801,0.00001064884,0.0005329977,0.000001424393],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9945215,0.0001899199,0.004238913,0.00005969595,0.000009779022,0.00001596737,0.0004668036,0.0000295652,0.0004678097],"genre_scores_gemma":[0.9916716,0.0002277307,0.00560845,0.0000226482,0.000003021565,0.00001370324,0.001090577,0.00003085758,0.001331381],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005710794,"threshold_uncertainty_score":0.001392663,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008140028690640153,"score_gpt":0.2129036946948605,"score_spread":0.2047636660042204,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}