{"id":"W2590124963","doi":"10.1038/srep42849","title":"A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix","year":2017,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Agarose; Biotinylation; Streptavidin; Sepharose; Affinity chromatography; Fusion protein; Chemistry; Tandem affinity purification; Chromatography; Recombinant DNA; Biochemistry; Biotin; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000577107,0.00007705179,0.000131024,0.00005277623,0.0002822023,0.00005605033,0.00009437883,0.0001063608,0.000001975624],"category_scores_gemma":[0.0006222592,0.00006843368,0.0000523471,0.00008063383,0.000152256,0.000009135121,0.00008828737,0.00002269287,2.700016e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007005029,"about_ca_system_score_gemma":0.00005128895,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002392216,"about_ca_topic_score_gemma":0.00001866884,"domain_scores_codex":[0.9990327,0.00001892107,0.0003133617,0.0004106725,0.0001206684,0.0001036667],"domain_scores_gemma":[0.9985569,0.00001165917,0.0005024958,0.0006124075,0.0002770735,0.00003952278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009421206,0.0001122663,0.002472696,0.0001842216,0.00003501816,0.00000139387,0.0002800747,0.001325781,0.9916243,0.00003002763,0.003166622,0.0006733621],"study_design_scores_gemma":[0.0002348157,0.0002382415,0.0009146243,0.00002202169,0.00004971159,0.0000134345,0.0002405987,0.002086311,0.9880643,0.0002935657,0.007715859,0.0001264696],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9933017,0.0004819086,0.004882196,0.00002049673,0.0002722418,0.0006946792,0.00002125787,0.00000621226,0.0003193366],"genre_scores_gemma":[0.9935924,0.00005185318,0.004597224,0.000002289127,0.00002353944,0.0000205968,0.0002229634,0.00000630004,0.001482882],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004549236,"threshold_uncertainty_score":0.2790645,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01473643782676434,"score_gpt":0.3013849694486699,"score_spread":0.2866485316219056,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}