{"id":"W2590124963","doi":"10.1038/srep42849","title":"A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix","year":2017,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Agarose; Biotinylation; Streptavidin; Sepharose; Affinity chromatography; Fusion protein; Chemistry; Tandem affinity purification; Chromatography; Recombinant DNA; Biochemistry; Biotin; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003991604,0.00117142,0.0005053963,0.0005859905,0.0003412288,0.000432689,0.0007692933,0.0007598056,0.001233112],"category_scores_gemma":[0.0003805865,0.0005435342,0.0005093417,0.0002724703,0.0002670257,0.0004211531,0.0007934626,0.001279022,0.001584995],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003124418,"about_ca_system_score_gemma":0.0003874737,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003354598,"about_ca_topic_score_gemma":0.0008186015,"domain_scores_codex":[0.999299,0.00009629998,0.00005723329,0.0001581501,0.00031398,0.00007528529],"domain_scores_gemma":[0.999764,0.00004084673,0.00004704883,0.00004850553,0.00005906797,0.0000404622],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000144044,0.00001685102,0.0000435465,0.0000745763,0.00001013071,0.00004877631,0.00001450587,0.00003364813,0.9962581,0.0001335596,0.00008717688,0.003264759],"study_design_scores_gemma":[0.000008301621,0.0001323118,0.0004627558,0.00000825121,0.0000148944,0.0007271391,0.00001471538,0.0006924759,0.9899834,0.0001210949,0.007817019,0.00001768009],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2888819,0.006763435,0.6940853,0.0006298763,0.0004477893,0.0009817473,0.000739886,0.002661544,0.004808558],"genre_scores_gemma":[0.4539841,0.004418986,0.5287635,0.0003587064,0.00008121901,0.0008716687,0.001943472,0.0002343967,0.009344032],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001233112,"threshold_uncertainty_score":0.004125118,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01473643782676434,"score_gpt":0.3013849694486699,"score_spread":0.2866485316219056,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}