{"id":"W2591012359","doi":"10.1038/srep43135","title":"Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families","year":2017,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Peptidase Inhibition and Analysis","field":"Medicine","cited_by":67,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"National Institute of General Medical Sciences; National Institutes of Health; Versus Arthritis; Politechnika Wrocławska; Fundacja na rzecz Nauki Polskiej; European Commission","keywords":"Proteases; Förster resonance energy transfer; Amino acid; Protease; Fluorophore; Substrate (aquarium); Chemistry; Caspase; Lysine; Cysteine; Biochemistry; Matrix metalloproteinase; Serine; Enzyme; Fluorescence; Biology; Apoptosis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001149022,0.000151429,0.0002565823,0.0001439605,0.0009435691,0.0004482442,0.00008503269,0.00005253766,0.00005753013],"category_scores_gemma":[0.0004673805,0.0000948858,0.00009432086,0.0002032578,0.001201481,0.0003047368,0.0001051157,0.0001727919,0.00001556448],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004399573,"about_ca_system_score_gemma":0.0001646793,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000678588,"about_ca_topic_score_gemma":0.0005006684,"domain_scores_codex":[0.9982435,0.00006552345,0.0003654128,0.0006109354,0.0004424626,0.0002722123],"domain_scores_gemma":[0.9983794,0.00002718777,0.0003161538,0.000950622,0.0001814916,0.0001451222],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"observational","study_design_gemma":"observational","study_design_scores_codex":[0.0001873815,0.0005455181,0.5566239,0.0001925399,0.00009738945,0.01309917,0.003420729,0.00002069714,0.4084763,0.0004614543,0.01236149,0.004513415],"study_design_scores_gemma":[0.0006568611,0.00005576793,0.5624422,0.0008678532,0.0001009065,0.0003059203,0.007867706,0.00101697,0.4216231,0.002114155,0.002626106,0.0003223846],"study_design_candidate":"observational","study_design_consensus":"observational","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9947294,0.00002838155,0.000009301544,0.001763014,0.0002657256,0.0005938141,0.00001303613,0.00003863557,0.002558681],"genre_scores_gemma":[0.9944339,0.00001140597,0.0002042875,0.00003504197,0.00007459352,0.00002928724,0.0000393861,0.000007501021,0.005164532],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01314679,"threshold_uncertainty_score":0.7257266,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02961780243803619,"score_gpt":0.2676160227784712,"score_spread":0.2379982203404351,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}