{"id":"W2592890495","doi":"10.1038/nprot.2017.006","title":"Simple multiplexed PCR-based barcoding of DNA for ultrasensitive mutation detection by next-generation sequencing","year":2017,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Cancer Genomics and Diagnostics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":137,"is_retracted":false,"has_abstract":false,"ca_institutions":"Ontario Institute for Cancer Research","funders":"National Cancer Institute","keywords":"Computational biology; Amplicon; Biology; DNA sequencing; DNA; DNA barcoding; Polymerase chain reaction; DNA microarray; Genetics; Gene; Evolutionary biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001618382,0.001413251,0.001098321,0.001606877,0.0005795142,0.00130226,0.002026868,0.0015873,0.003362844],"category_scores_gemma":[0.002295424,0.001479452,0.0009399031,0.0006041356,0.001030116,0.001178535,0.001801939,0.002451143,0.002871067],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006321082,"about_ca_system_score_gemma":0.001011219,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004219607,"about_ca_topic_score_gemma":0.002011004,"domain_scores_codex":[0.9971271,0.0003970886,0.0001896632,0.0009828758,0.001028323,0.0002748593],"domain_scores_gemma":[0.998709,0.0004718583,0.0001695986,0.0002654604,0.0002156119,0.0001684931],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000978034,0.00008209368,0.0002944747,0.0001348232,0.00002398774,0.00005406907,0.00005016377,0.0003443236,0.9787331,0.001565007,0.001254202,0.01736604],"study_design_scores_gemma":[0.00002416915,0.000188518,0.000690423,0.00002233255,0.00003604138,0.0002837192,0.00001841863,0.008008478,0.9798466,0.000998077,0.009832261,0.00005089217],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07613569,0.002455661,0.9067481,0.0005202977,0.0007733119,0.0009822636,0.002868694,0.006199745,0.003316136],"genre_scores_gemma":[0.1613238,0.001270336,0.8214792,0.0008489559,0.0001477108,0.001370209,0.004343834,0.0004840114,0.008731995],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003362844,"threshold_uncertainty_score":0.01124978,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04159425656903945,"score_gpt":0.3265408074719733,"score_spread":0.2849465509029339,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}