{"id":"W2594874402","doi":"10.1002/pro.3155","title":"Structural and functional characterization of a ubiquitin variant engineered for tight and specific binding to an alpha‐helical ubiquitin interacting motif","year":2017,"lang":"en","type":"article","venue":"Protein Science","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lunenfeld-Tanenbaum Research Institute; University of Toronto; Mount Sinai Hospital","funders":"Canadian Institutes of Health Research","keywords":"Ubiquitin; Proteome; Binding selectivity; Phage display; Computational biology; Protein–protein interaction; Motif (music); Ubiquitin ligase; Biology; Plasma protein binding; Structural motif; Yeast; Cell biology; Chemistry; Genetics; Biochemistry; Gene; Physics; Peptide","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001498911,0.0002416345,0.0001343554,0.0001319809,0.0001794792,0.0002218187,0.0002529161,0.000230291,0.0004653836],"category_scores_gemma":[0.0001430012,0.00009633592,0.000171941,0.0001777456,0.0002149617,0.0000919532,0.0001240776,0.0003350031,0.0002057891],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003892516,"about_ca_system_score_gemma":0.0001869843,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002231267,"about_ca_topic_score_gemma":0.001605575,"domain_scores_codex":[0.9999186,0.00001206357,0.000005934563,0.00001338326,0.00003073632,0.00001919867],"domain_scores_gemma":[0.9999174,0.00001511023,0.00001887843,0.000009917171,0.00001838942,0.00002039491],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004304288,0.00003097287,0.0002840839,0.00000984718,0.000004593391,0.00006005049,0.0000131287,0.0002558793,0.9983056,0.0001138773,0.00003425465,0.0008446776],"study_design_scores_gemma":[0.000007957329,0.0001110844,0.004005368,0.000002443115,0.00001256496,0.000294778,0.00002962642,0.002243058,0.9914762,0.00002610733,0.00178449,0.00000635157],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973084,0.0002107767,0.001795037,0.00002975878,0.000006446205,0.000009375471,0.0002485662,0.00001684133,0.0003749133],"genre_scores_gemma":[0.9953337,0.0001830632,0.002101203,0.00002251537,0.000002480691,0.00000750552,0.001015718,0.00002764474,0.001306082],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002231267,"threshold_uncertainty_score":0.004436493,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03281252052556446,"score_gpt":0.2797219305395968,"score_spread":0.2469094100140324,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}