{"id":"W2596341518","doi":"10.1093/biolreprod/83.s1.240","title":"How to Isolate Polysomal RNA to Study Mammalian Early Development.","year":2010,"lang":"en","type":"article","venue":"Biology of Reproduction","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"","keywords":"Biology; Polysome; RNA; Blastocyst; Messenger RNA; Cell biology; Oocyte; Translation (biology); Embryo; Population; Ribosome; Transcriptome; Computational biology; Embryogenesis; Genetics; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009439915,0.001075818,0.0008389337,0.001326704,0.0005462122,0.0008027748,0.0006437292,0.001070974,0.003205721],"category_scores_gemma":[0.0009548937,0.000402299,0.0007014728,0.0006256662,0.0008265238,0.001033532,0.0005837844,0.001931826,0.008141984],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002732732,"about_ca_system_score_gemma":0.0008670853,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007560988,"about_ca_topic_score_gemma":0.001743298,"domain_scores_codex":[0.9996096,0.00008886675,0.00003633988,0.0001056163,0.0001238901,0.00003557751],"domain_scores_gemma":[0.999402,0.0001549065,0.00005585281,0.0001315293,0.0001835961,0.00007201291],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0000413895,0.00004625494,0.0005251191,0.001117982,0.00003288273,0.0002275849,0.00009880857,0.0001259404,0.9527138,0.002117635,0.0015778,0.04137477],"study_design_scores_gemma":[0.00006324063,0.0004267922,0.007267878,0.0003746599,0.0002035792,0.002794119,0.0002922009,0.002481273,0.6880187,0.007494113,0.2904787,0.0001046578],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.0405221,0.05191431,0.8723639,0.004906062,0.0035248,0.002800304,0.005279586,0.002296224,0.01639268],"genre_scores_gemma":[0.06669727,0.04195172,0.8589692,0.002750226,0.0008294486,0.003237421,0.00767209,0.001039104,0.01685354],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003205721,"threshold_uncertainty_score":0.01072419,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01006734149952705,"score_gpt":0.2703048906284118,"score_spread":0.2602375491288847,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}