{"id":"W2605495829","doi":"10.1101/127837","title":"Investigation of protein synthesis in Drosophila larvae using puromycin labelling","year":2017,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Neurobiology and Insect Physiology Research","field":"Neuroscience","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"Alberta Children's Hospital; University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Puromycin; Protein biosynthesis; Biology; Messenger RNA; Cell biology; Immunostaining; Molecular biology; Zinc finger; Biochemistry; Transcription factor; Gene; Immunohistochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001711523,0.000351912,0.0001939075,0.0004043161,0.0001955971,0.0002446502,0.0002448318,0.0003055024,0.00120775],"category_scores_gemma":[0.0001248545,0.0001821409,0.0001684384,0.0001542574,0.0002552485,0.000224322,0.0001865218,0.000669474,0.000455082],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003842054,"about_ca_system_score_gemma":0.0001329724,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007619609,"about_ca_topic_score_gemma":0.00124795,"domain_scores_codex":[0.9999008,0.00001688826,0.00000929923,0.00003165547,0.00002780021,0.00001349955],"domain_scores_gemma":[0.9997759,0.00008448232,0.00005875983,0.00002464646,0.00002807723,0.00002800871],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000009570063,0.000002644273,0.00009420502,0.00001963351,0.000001575679,0.00001123759,0.000004861592,0.0000169745,0.9994743,0.00002317971,0.00001002747,0.0003317924],"study_design_scores_gemma":[0.000002842353,0.0000436857,0.003188103,0.00000487361,0.000005055338,0.00004450299,0.000007993312,0.0003266086,0.9952664,0.0000155556,0.001091749,0.000002532141],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9424561,0.005764833,0.04318329,0.0002029517,0.00008665435,0.0001932139,0.002222113,0.0004561569,0.005434754],"genre_scores_gemma":[0.9462647,0.003079683,0.0400805,0.0001529262,0.00003301923,0.0002571566,0.001718934,0.000204285,0.008208691],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00120775,"threshold_uncertainty_score":0.004040301,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06381754555057492,"score_gpt":0.2735518484786586,"score_spread":0.2097343029280837,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}