{"id":"W2610548715","doi":"10.1007/978-1-4939-6877-0_13","title":"Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid Luminescent DNA Precipitation Assay","year":2017,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Immune Cell Function and Interaction","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Institut National Du Cancer; Ligue Contre le Cancer; Institute of Cancer Research; Fondation pour la Recherche Médicale; Agence Nationale de la Recherche","keywords":"Chromatin immunoprecipitation; Transfection; Biology; Immunoprecipitation; Transcription factor; Cell biology; Chromatin; Molecular biology; Effector; Gene expression; Promoter; Gene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001145532,0.001377319,0.001366804,0.001232323,0.001701048,0.0009610371,0.001453136,0.0008620696,0.006096178],"category_scores_gemma":[0.0004865985,0.0008780165,0.001618658,0.001681725,0.001088248,0.0005351211,0.0007375326,0.004593063,0.003063233],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001157628,"about_ca_system_score_gemma":0.001173601,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003769623,"about_ca_topic_score_gemma":0.007882171,"domain_scores_codex":[0.9976447,0.00041041,0.0002671187,0.0005382964,0.0006219745,0.0005174357],"domain_scores_gemma":[0.9994928,0.0001496475,0.00005214746,0.0001087576,0.0001379601,0.00005866447],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009399396,0.00006014029,0.00008351309,0.00008740911,0.00001338912,0.00001727792,0.00003985226,0.00005505967,0.9983474,0.0001604777,0.0001726077,0.0008687309],"study_design_scores_gemma":[0.00003888443,0.00007790564,0.001050521,0.000006357596,0.00003026899,0.00005914879,0.00002485548,0.0007139909,0.9947668,0.00007742442,0.003144047,0.00000985722],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6639772,0.006487363,0.2941671,0.001554451,0.0009495947,0.001667995,0.0119246,0.003723892,0.01554783],"genre_scores_gemma":[0.6528165,0.005558913,0.2595093,0.00165643,0.0002595884,0.0059272,0.02993158,0.001263444,0.04307703],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.006096178,"threshold_uncertainty_score":0.02039379,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04465088792206846,"score_gpt":0.3839573984008114,"score_spread":0.3393065104787429,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}