{"id":"W2610847176","doi":"10.1021/acschembio.7b00203","title":"Iron Uptake Oxidoreductase (IruO) Uses a Flavin Adenine Dinucleotide Semiquinone Intermediate for Iron-Siderophore Reduction","year":2017,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Porphyrin Metabolism and Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; University of British Columbia","keywords":"Siderophore; Flavin adenine dinucleotide; Oxidoreductase; Cofactor; Semiquinone; Chemistry; Flavin group; Nicotinamide adenine dinucleotide phosphate; Ferrichrome; Reductase; Enzyme kinetics; Biochemistry; Stereochemistry; Flavoprotein; NAD+ kinase; Enzyme; Active site; Oxidase test; Escherichia coli; Quinone","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002036426,0.0004927642,0.0003270713,0.0002242059,0.0001850159,0.0003441392,0.0002636485,0.0005484696,0.0002886213],"category_scores_gemma":[0.0002024882,0.0001538838,0.0002829962,0.000183063,0.0002205426,0.0002481615,0.0002600518,0.0004167894,0.0003310851],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002261067,"about_ca_system_score_gemma":0.0001429819,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007696534,"about_ca_topic_score_gemma":0.0006283153,"domain_scores_codex":[0.9997911,0.00002473792,0.00001133958,0.00006620111,0.00006873697,0.00003788198],"domain_scores_gemma":[0.9998542,0.00002311174,0.00004695329,0.00001032979,0.00003528289,0.00003012046],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003734732,0.00002635357,0.0008423371,0.0000556118,0.000008316879,0.0000830772,0.00002473437,0.00007580658,0.9949017,0.0001180717,0.00008318169,0.003743553],"study_design_scores_gemma":[0.00001025682,0.0003114401,0.008540925,0.00001183503,0.00001548799,0.001044145,0.00007291088,0.00149828,0.9808127,0.0002712522,0.007398501,0.00001226606],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.962819,0.008707096,0.02451677,0.0002987127,0.00008104964,0.00006193041,0.0004242834,0.0004079019,0.002683285],"genre_scores_gemma":[0.9865383,0.001859034,0.008258194,0.0001104518,0.00001598556,0.00001457583,0.0006594689,0.00002022229,0.002523658],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007696534,"threshold_uncertainty_score":0.001640558,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01411262432798135,"score_gpt":0.2804195700009527,"score_spread":0.2663069456729714,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}