{"id":"W2614393829","doi":"10.1016/j.bbrc.2017.05.106","title":"Thiol-reactive drug substrates of human P-glycoprotein label the same sites to activate ATPase activity in membranes or dodecyl maltoside detergent micelles","year":2017,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"P-glycoprotein; Chemistry; Binding site; Micelle; Mutant; Membrane; Biochemistry; Thiol; Verapamil; Substrate (aquarium); Rhodamine 123; Transmembrane protein; Multiple drug resistance; Biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004304168,0.0004360803,0.0003280375,0.0001920511,0.0002828813,0.0004684607,0.0003548925,0.0006188322,0.002040406],"category_scores_gemma":[0.0003736412,0.0002905645,0.0003627356,0.0003342894,0.0003559887,0.0005518105,0.0002011637,0.0008653319,0.000472315],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005072919,"about_ca_system_score_gemma":0.000376697,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008276323,"about_ca_topic_score_gemma":0.00124714,"domain_scores_codex":[0.999756,0.00007931674,0.00001808949,0.00004866727,0.00003805825,0.00005983272],"domain_scores_gemma":[0.9997743,0.00008754481,0.00004032981,0.00003697708,0.00002601342,0.00003492023],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002685307,0.00003490564,0.0001347192,0.00004124516,0.00001292239,0.00004438535,0.00003245849,0.00005268282,0.998384,0.0003762568,0.00003625426,0.0005817097],"study_design_scores_gemma":[0.00001811942,0.0001566089,0.0003876375,0.000002366782,0.00001193952,0.00008026027,0.00001436681,0.0003820494,0.9983536,0.00004937285,0.0005411262,0.000002564105],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.990804,0.0009918575,0.005718708,0.0001714978,0.0000736952,0.00004749306,0.0001444107,0.00006252802,0.001985874],"genre_scores_gemma":[0.9888794,0.001011834,0.006589794,0.00008121106,0.00002627987,0.00004073669,0.0005146342,0.00004326084,0.00281284],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002040406,"threshold_uncertainty_score":0.006825864,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1413701122091389,"score_gpt":0.4177019147043377,"score_spread":0.2763318024951988,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}