{"id":"W2617225800","doi":"10.3791/55598","title":"Voltage-clamp Fluorometry in <em>Xenopus</em> Oocytes Using Fluorescent Unnatural Amino Acids","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"","keywords":"Xenopus; Transfer RNA; Fluorescence; Biophysics; Biochemistry; Voltage clamp; Amino acid; Intracellular; Chemistry; Biology; RNA; Membrane potential; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004119906,0.0004111209,0.0006082247,0.0002585014,0.0002705069,0.0002344556,0.0008044619,0.0003601844,0.0001354482],"category_scores_gemma":[0.000187553,0.0003426731,0.000349021,0.0001137304,0.0001332525,0.00005862299,0.000312406,0.0003272791,0.000006476268],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001315996,"about_ca_system_score_gemma":0.0001650419,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001982231,"about_ca_topic_score_gemma":0.000009729773,"domain_scores_codex":[0.9974563,0.0001192981,0.0008345462,0.0004146418,0.0006133434,0.0005618397],"domain_scores_gemma":[0.9979368,0.00001270612,0.0008705325,0.0006769609,0.0002363613,0.0002666621],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000676065,0.0003187045,0.004348455,0.00002535085,0.0001559506,0.0001151423,0.0004490244,0.000004839046,0.9911746,0.00002045379,0.0003935193,0.002317839],"study_design_scores_gemma":[0.004385561,0.0005093173,0.01926525,0.0001378117,0.00008922312,0.0001737789,0.0003110604,0.00003602976,0.9699967,0.000009626755,0.004691883,0.0003937831],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.994901,0.002442896,0.0001236504,0.00002259385,0.002071594,0.0002294223,0.00001464072,0.000008520887,0.0001856612],"genre_scores_gemma":[0.9954352,0.0002094239,0.002601661,0.0001430223,0.00107303,0.000007314291,0.00002839502,0.00005846468,0.0004434696],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02117799,"threshold_uncertainty_score":0.9999025,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02505351622437599,"score_gpt":0.3729197173274518,"score_spread":0.3478662011030759,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}