{"id":"W2625186074","doi":"10.1016/j.jbiotec.2017.06.009","title":"Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch","year":2017,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":70,"is_retracted":false,"has_abstract":false,"ca_institutions":"National Research Council Canada; Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; National Research Council Canada; Servier","keywords":"Chinese hamster ovary cell; Recombinant DNA; Plasmid; Biology; Fusion protein; Gene; Transfection; Molecular biology; Green fluorescent protein; Cell culture; Expression vector; Gene expression; Cell biology; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002422169,0.0001315495,0.0002105005,0.00005622162,0.0003988367,0.00008620477,0.0003297851,0.00028351,0.00001868937],"category_scores_gemma":[0.0002230765,0.00008716356,0.00009898566,0.00003262351,0.0003211853,0.00001641965,0.0002032498,0.0002357835,0.000001935565],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001682503,"about_ca_system_score_gemma":0.00007976909,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001039834,"about_ca_topic_score_gemma":0.00001394796,"domain_scores_codex":[0.999157,0.0000646738,0.0002721592,0.0002168375,0.0001069559,0.0001823632],"domain_scores_gemma":[0.998719,0.000006209419,0.0006371726,0.0005002666,0.00008233524,0.00005502739],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004825078,0.00004472192,0.000292785,0.00001040871,0.00006384704,0.000005839517,0.00001716231,0.00005075316,0.9962347,0.00002825399,0.0007443259,0.002024705],"study_design_scores_gemma":[0.001332549,0.0002467202,0.0005326597,0.00003722272,0.00004225253,0.00009305693,0.00004401997,0.00007118518,0.9891897,0.0007991684,0.007502916,0.0001085552],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9936487,0.003042034,0.0006353759,0.00176997,0.00056254,0.0002651924,0.00001402496,0.000008493824,0.00005368465],"genre_scores_gemma":[0.9969881,0.001339076,0.0009314922,0.00007230552,0.0005163303,0.000009738023,0.000003070741,0.00001574871,0.000124196],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007044996,"threshold_uncertainty_score":0.3554427,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01212697107458043,"score_gpt":0.2450345320967561,"score_spread":0.2329075610221757,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}