{"id":"W2625973567","doi":"10.1186/s13568-017-0425-y","title":"An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species","year":2017,"lang":"ca","type":"article","venue":"AMB Express","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"National Key Research and Development Program of China; National Natural Science Foundation of China; National Science Foundation","keywords":"Pseudomonas stutzeri; Biology; Mutagenesis; Genome; Genome editing; Bacterial genome size; Bacillus subtilis; Genetics; Plasmid; Escherichia coli; Mutant; Computational biology; DNA; Bacteria; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001387901,0.001098734,0.0009554701,0.0009351265,0.0006032331,0.0005536895,0.0009615032,0.0008239155,0.002444192],"category_scores_gemma":[0.001210144,0.0006663415,0.000789501,0.0007480647,0.0003781309,0.0006860184,0.0007804939,0.002085353,0.001818579],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002822678,"about_ca_system_score_gemma":0.0006486396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005156278,"about_ca_topic_score_gemma":0.00104412,"domain_scores_codex":[0.9981571,0.0004559493,0.0002747895,0.0002971603,0.0006550166,0.000159966],"domain_scores_gemma":[0.9993042,0.0001986118,0.0001270396,0.0001924197,0.0001302363,0.00004763738],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005060912,0.00003592305,0.00007824937,0.0001498502,0.00001245057,0.0001001499,0.00003428332,0.00009861027,0.9888961,0.0003139368,0.000456772,0.009773014],"study_design_scores_gemma":[0.00002081444,0.0001792301,0.0009841317,0.00002844947,0.00004115729,0.001246523,0.00001864755,0.001076271,0.9747595,0.0002200094,0.02137745,0.00004781446],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1199333,0.002630545,0.8649163,0.0003533874,0.0003522901,0.002014929,0.002201501,0.002940311,0.004657355],"genre_scores_gemma":[0.1877919,0.004959117,0.7779038,0.0002629899,0.00006540955,0.002870494,0.008734691,0.001181188,0.01623042],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002444192,"threshold_uncertainty_score":0.008176565,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01798565865175131,"score_gpt":0.3281747479184165,"score_spread":0.3101890892666652,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}