{"id":"W2625973567","doi":"10.1186/s13568-017-0425-y","title":"An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species","year":2017,"lang":"ca","type":"article","venue":"AMB Express","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"National Key Research and Development Program of China; National Natural Science Foundation of China; National Science Foundation","keywords":"Pseudomonas stutzeri; Biology; Mutagenesis; Genome; Genome editing; Bacterial genome size; Bacillus subtilis; Genetics; Plasmid; Escherichia coli; Mutant; Computational biology; DNA; Bacteria; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003073796,0.0003294665,0.0004375447,0.00006414902,0.0001623863,0.0001114161,0.0009351633,0.0002837475,0.0001334999],"category_scores_gemma":[0.0003174526,0.0003614047,0.0001721835,0.00003274138,0.0001328536,0.00002382513,0.0003536588,0.00009213728,0.000001587903],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000274707,"about_ca_system_score_gemma":0.00005984203,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001160395,"about_ca_topic_score_gemma":0.000538001,"domain_scores_codex":[0.9982142,0.00006397156,0.0004645873,0.0005718498,0.0001679126,0.000517444],"domain_scores_gemma":[0.9979307,0.00003322155,0.0003255747,0.001461139,0.0001285261,0.0001208514],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006426272,0.000142908,0.0005436473,0.0007384919,0.00005773882,0.000005000479,0.0007540005,0.0001310891,0.9949031,0.000008138581,0.0005424169,0.001530815],"study_design_scores_gemma":[0.004663825,0.000705711,0.021743,0.0001486106,0.00003182359,0.000001740553,0.0002126714,0.0001356962,0.9309427,0.0000211727,0.04096259,0.0004304576],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9099894,0.0004576702,0.02185215,0.0001366263,0.0008389931,0.06471926,0.0006954159,0.00002652436,0.001283934],"genre_scores_gemma":[0.9434092,0.00006314771,0.002227873,0.00001947417,0.002780805,0.05088882,0.0001185611,0.00007212975,0.0004199756],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06396042,"threshold_uncertainty_score":0.9998838,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01798565865175131,"score_gpt":0.3281747479184165,"score_spread":0.3101890892666652,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}