{"id":"W2725943195","doi":"10.1002/bit.26367","title":"Intracellular characterization of Gag VLP production by transient transfection of HEK 293 cells","year":2017,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Secretaría de Estado de Investigación, Desarrollo e Innovación; National Research Council Canada; Novo Nordisk","keywords":"Transfection; Internalization; Cytoplasm; Intracellular; Cell biology; Green fluorescent protein; Fluorescence microscope; Flow cytometry; Cell culture; HEK 293 cells; Confocal microscopy; Molecular biology; Biophysics; Chemistry; Cell; Biology; Fluorescence; Biochemistry; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000650766,0.0005384044,0.0009588115,0.0003519177,0.0002542856,0.0008779102,0.0005070608,0.0005599804,0.001101557],"category_scores_gemma":[0.0004605479,0.0002627564,0.0004765341,0.000625434,0.0003322762,0.0004317229,0.0003045564,0.001413813,0.001332197],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006808771,"about_ca_system_score_gemma":0.0003026105,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009058431,"about_ca_topic_score_gemma":0.0006653021,"domain_scores_codex":[0.9995311,0.00007645632,0.00005550006,0.00009043454,0.0001816907,0.00006476368],"domain_scores_gemma":[0.9997105,0.00008494908,0.0000361297,0.00004635634,0.00009959296,0.00002240924],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002047543,0.00001810908,0.00004526288,0.00004178239,0.000002896604,0.00002628411,0.00003086651,0.00006999602,0.9990305,0.00008380912,0.00003154751,0.0005985713],"study_design_scores_gemma":[0.000007254026,0.00007245546,0.0005812027,0.000004965552,0.00001063268,0.0000595311,0.00002074295,0.001978246,0.9952828,0.00003158641,0.001944415,0.000006045927],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7884911,0.003675022,0.1928109,0.000461315,0.0002649533,0.0007844224,0.005093845,0.001360611,0.007057954],"genre_scores_gemma":[0.8914379,0.002834033,0.08353438,0.0002337803,0.00005101064,0.0009519393,0.007728285,0.0004192272,0.01280939],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001101557,"threshold_uncertainty_score":0.004940152,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007069709364160107,"score_gpt":0.2232834509140268,"score_spread":0.2162137415498667,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}