{"id":"W2725943195","doi":"10.1002/bit.26367","title":"Intracellular characterization of Gag VLP production by transient transfection of HEK 293 cells","year":2017,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Secretaría de Estado de Investigación, Desarrollo e Innovación; National Research Council Canada; Novo Nordisk","keywords":"Transfection; Internalization; Cytoplasm; Intracellular; Cell biology; Green fluorescent protein; Fluorescence microscope; Flow cytometry; Cell culture; HEK 293 cells; Confocal microscopy; Molecular biology; Biophysics; Chemistry; Cell; Biology; Fluorescence; Biochemistry; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001486672,0.0000954837,0.0001233167,0.00007728422,0.00007078036,0.000006549234,0.0001293321,0.0002712589,0.00000324776],"category_scores_gemma":[0.00001580478,0.00009576132,0.0000343047,0.00004999436,0.0001878763,0.000006842595,0.0000237229,0.00009295656,3.916598e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005250167,"about_ca_system_score_gemma":0.00001178755,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006805325,"about_ca_topic_score_gemma":0.000007998861,"domain_scores_codex":[0.9994306,0.0000144872,0.0001502802,0.0002102782,0.00006311594,0.0001312432],"domain_scores_gemma":[0.9995511,0.000001668939,0.00007268078,0.0003149344,0.00003668069,0.00002290647],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002061179,0.00004172915,0.00008305987,0.00007204401,0.00004272396,3.766343e-7,0.00002083504,0.00003418724,0.9892027,0.00001437793,0.000009264356,0.01027254],"study_design_scores_gemma":[0.0002419237,0.0009020368,0.0005273026,0.00001870765,0.00001211515,0.000004853954,0.00001068232,0.0002081098,0.9969193,0.000003261173,0.001064103,0.00008764394],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9898804,0.0005039434,0.008803193,0.0004534079,0.0001381333,0.000172131,0.00002541214,0.00001745774,0.000005959662],"genre_scores_gemma":[0.997867,0.001862974,0.000121428,0.000003713243,0.00003892083,0.00000789491,0.00004307258,0.00001199717,0.00004302042],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0101849,"threshold_uncertainty_score":0.3905033,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007069709364160107,"score_gpt":0.2232834509140268,"score_spread":0.2162137415498667,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}