{"id":"W2734645439","doi":"10.1021/acs.biochem.7b00193","title":"Enhanced Binding Affinity via Destabilization of the Unbound State: A Millisecond Hydrogen–Deuterium Exchange Study of the Interaction between p53 and a Pleckstrin Homology Domain","year":2017,"lang":"en","type":"article","venue":"Biochemistry","topic":"Cancer-related Molecular Pathways","field":"Medicine","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"Natural Sciences and Engineering Research Council of Canada; Krembil Foundation","keywords":"Pleckstrin homology domain; Hydrogen–deuterium exchange; Deuterium; Chemistry; Hydrogen; Domain (mathematical analysis); Biophysics; Binding domain; Millisecond; Binding site; Stereochemistry; Biochemistry; Biology; Signal transduction; Organic chemistry; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001736582,0.0001497587,0.0001676062,0.00007097275,0.0002390084,0.0002069909,0.0002638381,0.0002056338,0.0009335819],"category_scores_gemma":[0.0001664457,0.0001361185,0.0000800796,0.00008797706,0.0003643456,0.0003047673,0.0001901203,0.0005608519,0.0001035655],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003018732,"about_ca_system_score_gemma":0.0001014725,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001557618,"about_ca_topic_score_gemma":0.001630967,"domain_scores_codex":[0.9999369,0.000006805232,0.000001782585,0.000013966,0.00001990983,0.00002061193],"domain_scores_gemma":[0.9999371,0.00003281086,0.00001114763,0.00000436784,0.000005139285,0.000009437531],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001706153,0.00004060729,0.0004158666,0.00004029428,0.0000121603,0.00009922713,0.0001029722,0.000401591,0.9966503,0.0004426186,0.0001051005,0.00151871],"study_design_scores_gemma":[0.00003975004,0.0002155147,0.004034592,0.000003632102,0.00001553711,0.0001741688,0.0001028852,0.0109628,0.9829172,0.0002251396,0.00129207,0.00001658874],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.996691,0.0003340657,0.001929852,0.00009686051,0.00001081804,0.000005716823,0.0000314201,0.00001587016,0.0008843786],"genre_scores_gemma":[0.9985034,0.0001798335,0.0005012664,0.00003772139,0.000004180902,0.000003496434,0.00003166361,0.000007454785,0.0007309234],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001557618,"threshold_uncertainty_score":0.003123164,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01979248252054027,"score_gpt":0.2776993608655607,"score_spread":0.2579068783450205,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}