{"id":"W2734645439","doi":"10.1021/acs.biochem.7b00193","title":"Enhanced Binding Affinity via Destabilization of the Unbound State: A Millisecond Hydrogen–Deuterium Exchange Study of the Interaction between p53 and a Pleckstrin Homology Domain","year":2017,"lang":"en","type":"article","venue":"Biochemistry","topic":"Cancer-related Molecular Pathways","field":"Medicine","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"Natural Sciences and Engineering Research Council of Canada; Krembil Foundation","keywords":"Pleckstrin homology domain; Hydrogen–deuterium exchange; Deuterium; Chemistry; Hydrogen; Domain (mathematical analysis); Biophysics; Binding domain; Millisecond; Binding site; Stereochemistry; Biochemistry; Biology; Signal transduction; Organic chemistry; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001797091,0.0001296834,0.0002455547,0.00003045267,0.0001675237,0.0000154259,0.0002126953,0.000122731,0.00002138237],"category_scores_gemma":[0.0001625723,0.00009367357,0.00007085459,0.0001073649,0.0002466879,0.00003984381,0.0002335461,0.0001942041,4.856338e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001097911,"about_ca_system_score_gemma":0.000103773,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001478758,"about_ca_topic_score_gemma":0.0001178876,"domain_scores_codex":[0.9990916,0.00006886306,0.0002810822,0.0002432323,0.0001759493,0.0001393212],"domain_scores_gemma":[0.9985788,0.00004120983,0.0004753603,0.0007700505,0.00008643217,0.00004815737],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000925394,0.0001315147,0.02089516,0.000191479,0.00007988212,0.000002264286,0.001004302,0.000002723808,0.9768974,3.687568e-7,0.00001335368,0.000688975],"study_design_scores_gemma":[0.001469788,0.0001514163,0.03379999,0.0001451249,0.0001196158,0.0000232339,0.0007442069,0.00001142116,0.9634049,0.00002701038,0.00002573136,0.00007754598],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987226,0.0001272357,0.0001474118,0.00007753429,0.0001555184,0.0005506634,0.00006660629,0.000007862424,0.0001445974],"genre_scores_gemma":[0.9997609,0.00001727308,0.00002760864,0.000009490087,0.0000528515,0.00002389422,0.00001645917,0.0000157521,0.00007578943],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01349252,"threshold_uncertainty_score":0.3819897,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01979248252054027,"score_gpt":0.2776993608655607,"score_spread":0.2579068783450205,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}