{"id":"W2735242611","doi":"10.1242/jeb.160176","title":"CRISPR-induced null alleles show that <i>Frost</i> protects <i>Drosophila melanogaster</i> reproduction after cold exposure","year":2017,"lang":"en","type":"article","venue":"Journal of Experimental Biology","topic":"Physiological and biochemical adaptations","field":"Environmental Science","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; Western University","funders":"Japan Society for the Promotion of Science; Natural Sciences and Engineering Research Council of Canada","keywords":"Biology; Drosophila melanogaster; Gene knockdown; RNA interference; Genetics; Gene; CRISPR; Allele; Null allele; Cell biology; Cas9; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001150408,0.0004811213,0.0002942173,0.0003116395,0.0002814909,0.0003682198,0.000510157,0.0005474021,0.006055578],"category_scores_gemma":[0.0001382533,0.0002648783,0.0005161714,0.000133653,0.0004129668,0.0002354324,0.0004216165,0.001176268,0.001709479],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004337212,"about_ca_system_score_gemma":0.0003065489,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002142245,"about_ca_topic_score_gemma":0.003650169,"domain_scores_codex":[0.9998055,0.00001510687,0.00002536856,0.00005921143,0.00005962201,0.00003520558],"domain_scores_gemma":[0.9997668,0.00003921861,0.00008188545,0.00003568574,0.00001627248,0.00006016426],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003254446,0.00001106808,0.0001569384,0.00003582329,0.000007570166,0.0001128038,0.0000140242,0.0000625004,0.9983254,0.0001990816,0.0001356749,0.000906497],"study_design_scores_gemma":[0.00002610294,0.00006761336,0.009857327,0.00001648885,0.00002376752,0.0006345471,0.00003847576,0.00137237,0.9794867,0.0001141925,0.008345329,0.00001701696],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9555093,0.0009708084,0.02316316,0.0005552029,0.0001920864,0.00008525993,0.005794243,0.001624886,0.01210514],"genre_scores_gemma":[0.9735082,0.0005128054,0.008258278,0.0003657843,0.00001594826,0.00007396135,0.003554415,0.0005320303,0.01317868],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006055578,"threshold_uncertainty_score":0.02025789,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03276727112066811,"score_gpt":0.2732199006608048,"score_spread":0.2404526295401366,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}