{"id":"W2737033486","doi":"10.1021/acs.analchem.7b01310","title":"Topological Dissection of the Membrane Transport Protein Mhp1 Derived from Cysteine Accessibility and Mass Spectrometry","year":2017,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Mass Spectrometry Techniques and Applications","field":"Chemistry","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"H2020 European Research Council; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; Hamburg Centre for Ultrafast Imaging; Leverhulme Trust; Biotechnology and Biological Sciences Research Council; Joachim Herz Stiftung; Wellcome Trust; National Institute of General Medical Sciences; Medical Research Council; Wellcome","keywords":"Chemistry; Cysteine; Mass spectrometry; Substrate (aquarium); Conformational change; Crystallography; Ion-mobility spectrometry; Stereochemistry; Enzyme; Chromatography; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001865257,0.0003418511,0.0001711022,0.000327367,0.0001874811,0.0003186135,0.0002721658,0.0002634937,0.0006413611],"category_scores_gemma":[0.0002691659,0.0001398382,0.0002182102,0.0002157045,0.0002915798,0.0002797193,0.0004113178,0.0005099783,0.0002362693],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003552833,"about_ca_system_score_gemma":0.0001510932,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004459462,"about_ca_topic_score_gemma":0.0004867217,"domain_scores_codex":[0.9999126,0.00001245479,0.000006296239,0.00002062063,0.00003326209,0.00001477318],"domain_scores_gemma":[0.9998305,0.00003368185,0.00006010822,0.00001715137,0.000027052,0.00003149419],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003569261,0.000003041293,0.0001942971,0.00002427557,0.000001819126,0.00002861387,0.00001409469,0.00003508185,0.9989414,0.00006745258,0.000009251731,0.0006449308],"study_design_scores_gemma":[0.000004986244,0.00007188787,0.007191578,0.000007561493,0.000008520689,0.0003778567,0.00006364431,0.001679243,0.9890742,0.0001283956,0.001383898,0.000008269632],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9846985,0.0004194795,0.01347853,0.00009137088,0.00001013673,0.00003511844,0.0004427174,0.0001082576,0.000715985],"genre_scores_gemma":[0.9806808,0.0006674873,0.01590351,0.00004099129,0.000009404128,0.00005547692,0.001325662,0.00005348715,0.001263314],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006413611,"threshold_uncertainty_score":0.002577782,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01663759611318595,"score_gpt":0.2797211697338386,"score_spread":0.2630835736206527,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}