{"id":"W2740617547","doi":"10.1039/c7ob01522d","title":"A hybrid molecule of a GFP chromophore analogue and cholestene as a viscosity-dependent and cholesterol-responsive fluorescent sensor","year":2017,"lang":"en","type":"article","venue":"Organic & Biomolecular Chemistry","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Japan Society for the Promotion of Science; Université Laval","keywords":"Chromophore; Chemistry; Green fluorescent protein; Fluorescence; Molecule; Viscosity; Photochemistry; Fluorescent protein; Biophysics; Biochemistry; Organic chemistry; Thermodynamics; Physics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002162133,0.0002029597,0.0002435329,0.000116041,0.00007759751,0.0001610655,0.000238483,0.0003296567,0.0003967565],"category_scores_gemma":[0.0001676926,0.000107711,0.0001263275,0.0001353277,0.0002223916,0.0002721499,0.0001821591,0.0003423059,0.0001298086],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003207979,"about_ca_system_score_gemma":0.0001541841,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003248398,"about_ca_topic_score_gemma":0.0005291268,"domain_scores_codex":[0.9998996,0.00001621019,0.00000556717,0.00003534808,0.00002689215,0.00001621644],"domain_scores_gemma":[0.9998994,0.00002376688,0.00002216351,0.00001117182,0.00001761591,0.00002579273],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001822065,0.000004284692,0.00004757887,0.0000105996,0.000001243096,0.0000192709,0.00000412078,0.00002796983,0.999373,0.00005301963,0.000004478216,0.0004362014],"study_design_scores_gemma":[0.00000781969,0.00007097994,0.0003766737,0.000001155207,0.000003736307,0.00009948203,0.000006447311,0.0005840505,0.9981761,0.00001311059,0.0006570718,0.000003350958],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9882579,0.0006446524,0.009586423,0.00008730264,0.00002571939,0.00003570879,0.0001119091,0.00007184019,0.001178616],"genre_scores_gemma":[0.9818408,0.0003205377,0.01575209,0.00004682927,0.000007799691,0.00003126805,0.0001455493,0.00001214852,0.001842816],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0003967565,"threshold_uncertainty_score":0.002327502,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005536302981329043,"score_gpt":0.2394693236746769,"score_spread":0.2339330206933478,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}