{"id":"W2745842388","doi":"10.1038/s41598-017-08813-1","title":"RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR","year":2017,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":74,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Animal and Plant Health Inspection Service; Agricultural Research Service; National Institute of Food and Agriculture; U.S. Department of Agriculture","keywords":"RNA interference; Gene knockdown; RNA silencing; Biology; Gene; Dicer; Gene silencing; Reference genes; RNA; Argonaute; Gene expression; Genetics; Cell biology","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001142187,0.001461374,0.0009421993,0.001092418,0.0006219744,0.0008009074,0.0005715929,0.0005575991,0.002142307],"category_scores_gemma":[0.0006239592,0.0008954036,0.0008974201,0.0009974189,0.0007742063,0.0005371642,0.000414773,0.001582408,0.001330693],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007025278,"about_ca_system_score_gemma":0.0007444272,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008057349,"about_ca_topic_score_gemma":0.002647144,"domain_scores_codex":[0.9986244,0.0001664995,0.0001785998,0.0005251681,0.0003629332,0.0001424115],"domain_scores_gemma":[0.9995542,0.0001118026,0.0001205813,0.00004418379,0.0001349611,0.00003415118],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004829283,0.00003863264,0.0001482929,0.00009591352,0.000006246699,0.00001609396,0.00004703438,0.0001046477,0.9978568,0.0001405815,0.00006229693,0.001435048],"study_design_scores_gemma":[0.00004833107,0.0004499358,0.004189784,0.00004736702,0.0001105291,0.000155433,0.00008336099,0.003714032,0.9823077,0.0002997648,0.008566706,0.00002705347],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5112609,0.005068484,0.4548323,0.0003627136,0.0004658233,0.003514035,0.01216849,0.002943738,0.009383464],"genre_scores_gemma":[0.2796242,0.003571535,0.6764449,0.0006908319,0.00009579704,0.009122644,0.01462703,0.00140748,0.01441562],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002142307,"threshold_uncertainty_score":0.007166743,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02469862702448101,"score_gpt":0.2900543886555913,"score_spread":0.2653557616311103,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}