{"id":"W2746351040","doi":"10.1016/j.jviromet.2017.08.008","title":"User-friendly Taqman probe coupled-insulated isothermal PCR (iiPCR) for rapid detection of emerging Ambystoma tigrinum virus (ATV) in western tiger salamanders ( Ambystoma mavortium ) on a compact, portable instrument","year":2017,"lang":"en","type":"article","venue":"Journal of Virological Methods","topic":"Animal Disease Management and Epidemiology","field":"Agricultural and Biological Sciences","cited_by":3,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canadian Food Inspection Agency; University of Lethbridge","funders":"","keywords":"Tiger salamander; Biology; Loop-mediated isothermal amplification; TaqMan; Iridovirus; Virology; Ranavirus; Bed bug; Dipstick; Polymerase chain reaction; Virus; Zoology; Larva; Ecology; Genetics; DNA; Gene; Anatomy","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00171168,0.001895141,0.001336502,0.001053874,0.0004302592,0.0006815916,0.001469318,0.0009123086,0.006545864],"category_scores_gemma":[0.001884523,0.001302886,0.0008800158,0.000689062,0.0006110315,0.0006936137,0.0007991479,0.001318591,0.004226011],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000428805,"about_ca_system_score_gemma":0.0006893149,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006348541,"about_ca_topic_score_gemma":0.002073353,"domain_scores_codex":[0.9965965,0.0006626532,0.0001814311,0.001311843,0.0009538186,0.0002937014],"domain_scores_gemma":[0.9990086,0.0004837922,0.0001082279,0.0001201685,0.0001829034,0.00009624482],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004342503,0.0001140751,0.002087635,0.0001888489,0.00002933842,0.0001036016,0.0001149518,0.0002577123,0.9740309,0.0003651683,0.00143171,0.02084174],"study_design_scores_gemma":[0.0001570744,0.002225264,0.01358152,0.00007359831,0.0001976474,0.001146375,0.0001172,0.01846435,0.9426701,0.0004609378,0.02080175,0.0001042007],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2278662,0.001611114,0.744655,0.0003749205,0.0006143711,0.001279603,0.006047983,0.01120447,0.006346312],"genre_scores_gemma":[0.2464251,0.0009257111,0.7147297,0.0008674136,0.0001932516,0.003220215,0.009594885,0.001053193,0.02299045],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.006545864,"threshold_uncertainty_score":0.02189815,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06211790841322673,"score_gpt":0.3463805631756785,"score_spread":0.2842626547624518,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}