{"id":"W2748309952","doi":"10.3791/55909","title":"Quantitative Immunofluorescence to Measure Global Localized Translation","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hôtel-Dieu de Québec; Université Laval","funders":"Canadian Institutes of Health Research; Université Laval","keywords":"Translation (biology); Messenger RNA; Translational regulation; Protein biosynthesis; Cell biology; Biology; Organogenesis; Computational biology; Molecular biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004436654,0.0001457863,0.0002397113,0.00003276682,0.000198967,0.00009854126,0.0004787718,0.0001084049,0.00003671673],"category_scores_gemma":[0.0002698909,0.0001247368,0.0001645107,0.00003482116,0.00004871329,0.00002000311,0.00005670949,0.00005173747,0.0000152168],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003106379,"about_ca_system_score_gemma":0.000082591,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002024993,"about_ca_topic_score_gemma":0.000002900861,"domain_scores_codex":[0.9988251,0.0001317603,0.0003636356,0.0001685863,0.0003329241,0.0001780263],"domain_scores_gemma":[0.9988625,0.000008061653,0.0004392191,0.0003385657,0.0002161204,0.0001355044],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002083149,0.00008590287,0.0002403883,0.000004272506,0.0001140638,0.000008021937,0.0001672018,0.00000581549,0.9885353,0.00022962,0.0003521373,0.008174078],"study_design_scores_gemma":[0.001746925,0.001006038,0.0005433235,0.00009023167,0.00002201995,0.00002287611,0.0001605242,0.00001422855,0.9892295,0.0002091529,0.006802221,0.0001529582],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8658479,0.004276127,0.1270925,0.0003566555,0.000786001,0.0003429841,0.00001357341,0.000006305524,0.001277975],"genre_scores_gemma":[0.9678195,0.0001237665,0.03168332,0.000147214,0.0001335984,0.00000979916,0.000002915143,0.00001495115,0.00006489368],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1019717,"threshold_uncertainty_score":0.508662,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05770352265854522,"score_gpt":0.4353777778063916,"score_spread":0.3776742551478464,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}