{"id":"W2748309952","doi":"10.3791/55909","title":"Quantitative Immunofluorescence to Measure Global Localized Translation","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hôtel-Dieu de Québec; Université Laval","funders":"Canadian Institutes of Health Research; Université Laval","keywords":"Translation (biology); Messenger RNA; Translational regulation; Protein biosynthesis; Cell biology; Biology; Organogenesis; Computational biology; Molecular biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002059127,0.001995678,0.001054974,0.0024655,0.0007536274,0.001014993,0.001660551,0.001313724,0.01047656],"category_scores_gemma":[0.0007410005,0.0007721588,0.0009405669,0.001660659,0.001008627,0.001127232,0.000857171,0.003610641,0.003799183],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001053739,"about_ca_system_score_gemma":0.0006218114,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007749729,"about_ca_topic_score_gemma":0.001510718,"domain_scores_codex":[0.99832,0.0003688533,0.0001071668,0.0005086454,0.0005056653,0.0001896987],"domain_scores_gemma":[0.9990799,0.0004087012,0.0000828598,0.0001663873,0.0002051933,0.0000568086],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004552038,0.00005753186,0.00008343442,0.0002987773,0.00001658812,0.00002734185,0.00004580947,0.0001340707,0.992243,0.001403761,0.0005388463,0.005105335],"study_design_scores_gemma":[0.00003816388,0.0002644726,0.001654116,0.00006115501,0.00006002979,0.0004216439,0.00004816537,0.006032998,0.9619072,0.0009445457,0.02852526,0.00004211645],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.06492514,0.009141826,0.9000109,0.0004814464,0.0008054245,0.0007953114,0.003574418,0.004157722,0.01610781],"genre_scores_gemma":[0.1928201,0.01254193,0.7550557,0.0006095372,0.0004020702,0.004609191,0.005252666,0.001466247,0.02724259],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01047656,"threshold_uncertainty_score":0.03504759,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05770352265854522,"score_gpt":0.4353777778063916,"score_spread":0.3776742551478464,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}