{"id":"W2749058681","doi":"10.1021/acsomega.7b00782","title":"DNA Aptamer–Target Binding Motif Revealed Using a Fluorescent Guanine Probe: Implications for Food Toxin Detection","year":2017,"lang":"en","type":"article","venue":"ACS Omega","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Agricultural Adaptation Council; Grain Farmers of Ontario","keywords":"Aptamer; Oligonucleotide; DNA; G-quadruplex; Guanine; Antiparallel (mathematics); Fluorescence; Chemistry; Biochemistry; Stacking; Biology; Biophysics; Molecular biology; Gene; Nucleotide","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000164571,0.0002762616,0.0002154928,0.0001303629,0.000130507,0.000184111,0.0002463761,0.0006701075,0.0006035158],"category_scores_gemma":[0.0002409482,0.0001167542,0.0001584486,0.0001542677,0.000305103,0.000232382,0.0001566043,0.000335702,0.0001969329],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002879089,"about_ca_system_score_gemma":0.0001713974,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000860236,"about_ca_topic_score_gemma":0.0009241253,"domain_scores_codex":[0.9998863,0.00002666926,0.000004305156,0.00003409609,0.0000265156,0.00002221904],"domain_scores_gemma":[0.9999161,0.00002057999,0.00002504629,0.000006304847,0.0000132288,0.0000187348],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001963717,0.000005606712,0.00006984574,0.00001114749,8.107333e-7,0.00002727795,0.000008814155,0.00004565345,0.9991565,0.00005517423,0.000008259603,0.0005911746],"study_design_scores_gemma":[0.00000482695,0.0001742363,0.0009848587,0.000002128547,0.000005054983,0.0001724038,0.00001634368,0.001295673,0.99672,0.00005544138,0.0005635362,0.000005381699],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.979685,0.001020014,0.01782511,0.000302289,0.00002073197,0.00002866832,0.00009811265,0.000141705,0.0008782182],"genre_scores_gemma":[0.9809421,0.0005689297,0.01718289,0.0001082514,0.000005064356,0.00002498409,0.000101553,0.00001034767,0.001055985],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000860236,"threshold_uncertainty_score":0.002088904,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03022474361350243,"score_gpt":0.3134304987537331,"score_spread":0.2832057551402307,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}