{"id":"W2756439426","doi":"10.1007/978-1-4939-7240-1_20","title":"Measuring Cyclic Diguanylate (c-di-GMP)-Specific Phosphodiesterase Activity Using the MANT-c-di-GMP Assay","year":2017,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Phosphodiesterase function and regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Canadian Institutes of Health Research","keywords":"Cyclic gmp; Phosphodiesterase; Chemistry; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.002155886,0.0004095421,0.0004330759,0.0001144803,0.0006756617,0.0001507663,0.0007251984,0.000478132,0.00002274996],"category_scores_gemma":[0.0005427559,0.0003215768,0.0002542844,0.0001204583,0.0005124682,0.00002005441,0.0005549032,0.0003933443,0.000007178472],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008045325,"about_ca_system_score_gemma":0.00007224523,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001423016,"about_ca_topic_score_gemma":0.00007736345,"domain_scores_codex":[0.9962688,0.001692954,0.0004252092,0.000855026,0.0001566049,0.0006013695],"domain_scores_gemma":[0.9976215,0.0001014191,0.0004118825,0.001646004,0.0001071606,0.0001120484],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001874322,0.00006804686,0.00856654,0.00001297468,0.00008595199,0.00002353675,0.0000621996,0.0004115427,0.9592457,0.0002824652,0.00002647453,0.03102711],"study_design_scores_gemma":[0.0009992104,0.0001208217,0.01943823,0.00003756151,0.00003720446,0.00006291384,0.00004254879,0.00197855,0.9587989,0.0008362241,0.01716975,0.0004780593],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4944879,0.00123237,0.5021663,0.0002647105,0.0009490463,0.000284857,0.00000908291,0.00002061283,0.0005851627],"genre_scores_gemma":[0.9346138,0.0003895559,0.06394403,0.0003147056,0.0002607811,0.00005988248,0.00004174972,0.00006583273,0.0003096538],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.4401259,"threshold_uncertainty_score":0.9999236,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07400311417006825,"score_gpt":0.3841469241812985,"score_spread":0.3101438100112303,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}