{"id":"W2757608129","doi":"10.3791/56373","title":"Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canadian Food Inspection Agency; University of Calgary","funders":"Alberta Agriculture and Forestry; Genome Canada; National Institutes of Health; Genome Alberta; University of Calgary","keywords":"Feces; Chronic wasting disease; Chemistry; Recombinant DNA; Microbiology; Thioflavin; Molecular biology; Biology; Chromatography; Biochemistry; Virology; Prion protein; Scrapie; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001519607,0.001085303,0.0008342142,0.0009340918,0.0003631366,0.0005363114,0.0007362064,0.00094565,0.0009620301],"category_scores_gemma":[0.001610745,0.000581139,0.0005907794,0.0006258016,0.0006322104,0.0004148724,0.0004040932,0.001278713,0.0007082563],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003036593,"about_ca_system_score_gemma":0.0003690182,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006470547,"about_ca_topic_score_gemma":0.001388941,"domain_scores_codex":[0.9979076,0.0006368668,0.000309312,0.0004177258,0.000582801,0.0001457028],"domain_scores_gemma":[0.998606,0.0005534034,0.0002724901,0.0001808918,0.000318979,0.00006817555],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002879231,0.00004104763,0.0002933982,0.00007514215,0.000004158195,0.00004482657,0.00004188725,0.00003173531,0.9983774,0.00002818091,0.00002887604,0.001004457],"study_design_scores_gemma":[0.000008732744,0.0003714022,0.003409971,0.00001860934,0.00003194993,0.0003058873,0.00002970774,0.001898107,0.9927564,0.00003970769,0.001109121,0.00002029694],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6744615,0.002905,0.3148072,0.0002286684,0.0003088246,0.001537116,0.001574373,0.001309825,0.002867365],"genre_scores_gemma":[0.4945866,0.002230705,0.4919635,0.0002679323,0.00006511468,0.001517001,0.003118402,0.0001587853,0.006091806],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001519607,"threshold_uncertainty_score":0.008036554,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02712645741637244,"score_gpt":0.4149029123430255,"score_spread":0.387776454926653,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}