{"id":"W2758601131","doi":"10.1016/j.pep.2017.09.015","title":"Expression, purification, and functional analysis of an antigen-targeting fusion protein composed of CD40 ligand and the C-terminal fragment of ovalbumin","year":2017,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Immunotherapy and Immune Responses","field":"Immunology and Microbiology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Dalhousie University; Izaak Walton Killam Health Centre; Nova Scotia Health Authority","funders":"","keywords":"Ovalbumin; Fusion protein; Affinity chromatography; Antigen; Molecular biology; Escherichia coli; Biology; CD40; Immune system; Ligand (biochemistry); Recombinant DNA; Chemistry; Biochemistry; Receptor; In vitro; Gene; Immunology; Cytotoxic T cell","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003188964,0.0003486869,0.0002571555,0.0002945367,0.0003680707,0.0003965514,0.0002199951,0.0002904141,0.0004427583],"category_scores_gemma":[0.0002230943,0.0001780337,0.0002888115,0.0005263381,0.0002875987,0.0003699795,0.0001947871,0.0006392922,0.0003494992],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007723987,"about_ca_system_score_gemma":0.0004492707,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002199003,"about_ca_topic_score_gemma":0.001434622,"domain_scores_codex":[0.9998337,0.00002470559,0.00001647019,0.00002753507,0.00005034479,0.00004729401],"domain_scores_gemma":[0.9998661,0.00002493206,0.00002735187,0.00001415733,0.00003169032,0.00003578512],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006143832,0.00003325703,0.0001296844,0.00001968181,0.000002894127,0.0000369865,0.00002822102,0.00007179012,0.999036,0.0001570427,0.00003210242,0.0003910041],"study_design_scores_gemma":[0.00001163133,0.0000691493,0.003549941,0.00000314749,0.00001982775,0.00030815,0.00004441096,0.001647324,0.9913573,0.0001022294,0.002881065,0.000005877101],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9924111,0.000694408,0.00530962,0.0002164508,0.00001675288,0.00003077875,0.0007598824,0.00004348956,0.0005174557],"genre_scores_gemma":[0.9861947,0.0008506812,0.006533378,0.00007092259,0.00001494898,0.00003641012,0.003231129,0.00004717539,0.003020672],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002199003,"threshold_uncertainty_score":0.005604208,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01493121454371922,"score_gpt":0.2536955377131922,"score_spread":0.238764323169473,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}