{"id":"W2758601131","doi":"10.1016/j.pep.2017.09.015","title":"Expression, purification, and functional analysis of an antigen-targeting fusion protein composed of CD40 ligand and the C-terminal fragment of ovalbumin","year":2017,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Immunotherapy and Immune Responses","field":"Immunology and Microbiology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Dalhousie University; Izaak Walton Killam Health Centre; Nova Scotia Health Authority","funders":"","keywords":"Ovalbumin; Fusion protein; Affinity chromatography; Antigen; Molecular biology; Escherichia coli; Biology; CD40; Immune system; Ligand (biochemistry); Recombinant DNA; Chemistry; Biochemistry; Receptor; In vitro; Gene; Immunology; Cytotoxic T cell","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001114339,0.0001703483,0.0004489708,0.0002089523,0.0006555886,0.0000355456,0.0002109145,0.0001882617,0.00006955268],"category_scores_gemma":[0.0002174075,0.0001146313,0.00007696848,0.0001095576,0.0009303043,0.0002382859,0.0001029565,0.0001504211,6.625525e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000549229,"about_ca_system_score_gemma":0.00003587453,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001097206,"about_ca_topic_score_gemma":0.000002208322,"domain_scores_codex":[0.9982586,0.0005392058,0.0006322225,0.000320754,0.0001081017,0.0001411438],"domain_scores_gemma":[0.9979296,0.0001284991,0.00111627,0.0005922029,0.0002091532,0.00002425748],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00309719,0.0001816463,0.001589131,0.00009405571,0.0001500446,2.207388e-7,0.001502358,0.000002919563,0.984298,0.000504677,0.00001599586,0.008563756],"study_design_scores_gemma":[0.002090405,0.0001345556,0.1108803,0.0002495787,0.00009846462,0.000004895223,0.0004287628,0.0001330536,0.88513,0.000182695,0.0005577672,0.0001095048],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9896416,0.008196255,0.000911331,0.000236355,0.00004975781,0.0008908911,0.00002747031,0.00001096785,0.00003536374],"genre_scores_gemma":[0.9979913,0.0006391917,0.0008615066,0.000008365565,0.00001183826,0.0001043978,0.0001030045,0.00001007348,0.0002703499],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1092911,"threshold_uncertainty_score":0.5042324,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01493121454371922,"score_gpt":0.2536955377131922,"score_spread":0.238764323169473,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}