{"id":"W2763071761","doi":"10.1094/pdis-07-17-0968-re","title":"High Resolution DNA Melting Assays for Detection of <i>Rx1</i> and <i>Rx2</i> for High-Throughput Marker-Assisted Selection for Extreme Resistance to <i>Potato virus X</i> in Tetraploid Potato","year":2017,"lang":"en","type":"article","venue":"Plant Disease","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"123 Certification (Canada); University of Victoria; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada","keywords":"Biology; Primer (cosmetics); High Resolution Melt; Amplicon; Polymerase chain reaction; Agarose gel electrophoresis; Potato virus X; Gel electrophoresis; Molecular biology; DNA; Genetics; Gene; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001008914,0.001129158,0.001091773,0.001055676,0.0002911807,0.0004886015,0.0006387697,0.0009741984,0.001732352],"category_scores_gemma":[0.001478019,0.0006478383,0.0009781483,0.0006617687,0.0002668661,0.0004665913,0.0005144812,0.001579304,0.00161939],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003004619,"about_ca_system_score_gemma":0.000260021,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002296998,"about_ca_topic_score_gemma":0.000735603,"domain_scores_codex":[0.9986082,0.0002770387,0.0001612962,0.0004968906,0.0003450601,0.0001115505],"domain_scores_gemma":[0.999149,0.0002590621,0.0002403173,0.0001016148,0.0001726191,0.00007742162],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002111909,0.00001612296,0.0001247622,0.00003998551,0.000005603162,0.00001192456,0.0000225838,0.00005469792,0.9983523,0.00002328607,0.00002693095,0.001300795],"study_design_scores_gemma":[0.000009025133,0.0002015451,0.002635049,0.00001883847,0.00004439878,0.0001954887,0.00002933758,0.002089244,0.9929283,0.00005081697,0.001776846,0.00002115671],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.528188,0.003460984,0.4564664,0.0002699737,0.0001492165,0.0008863195,0.004207822,0.003150933,0.003220232],"genre_scores_gemma":[0.5050118,0.002645074,0.4687141,0.0003228992,0.00005187756,0.001978924,0.0111497,0.0007350481,0.009390606],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001732352,"threshold_uncertainty_score":0.0057953,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04584405699660078,"score_gpt":0.2626503301123842,"score_spread":0.2168062731157834,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}