{"id":"W2766433963","doi":"10.3791/56281","title":"High Resolution Fluorescent &lt;em&gt;In Situ&lt;/em&gt; Hybridization in &lt;em&gt;Drosophila&lt;/em&gt; Embryos and Tissues Using Tyramide Signal Amplification","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Developmental Biology and Gene Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"In situ hybridization; Biology; Molecular biology; Complementary DNA; Subcellular localization; Streptavidin; RNA; Horseradish peroxidase; Oligonucleotide; Messenger RNA; Cell biology; DNA; Biotin; Cytoplasm; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0009713061,0.0004279909,0.0005749702,0.0003388099,0.0004118757,0.0002097941,0.0003947984,0.0004628442,0.00003619521],"category_scores_gemma":[0.0001903716,0.0004256793,0.0001457938,0.0001628795,0.0001631634,0.0001271313,0.0002292612,0.0002147641,0.00001188845],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002893913,"about_ca_system_score_gemma":0.0001746401,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002169588,"about_ca_topic_score_gemma":0.0001159352,"domain_scores_codex":[0.9968127,0.000355095,0.001175572,0.0006174779,0.0005329686,0.0005061565],"domain_scores_gemma":[0.9978382,0.00002400981,0.001198385,0.000446302,0.0002959191,0.0001971795],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001082638,0.0003715157,0.002945103,0.00003273373,0.0001146588,0.00003162196,0.0006419566,0.0003340538,0.9905385,0.0001133873,0.0004850678,0.00330873],"study_design_scores_gemma":[0.004359971,0.0004801967,0.09626653,0.0003005358,0.00005713499,0.000111738,0.0002241943,0.001166197,0.8939102,0.0003072754,0.002291363,0.000524696],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9923468,0.004286492,0.002043273,0.0001250549,0.0006129277,0.0004708694,0.00001760294,0.00001412873,0.00008291777],"genre_scores_gemma":[0.993695,0.001143291,0.004022247,0.0001026274,0.000435997,0.00002132322,0.0003403943,0.00004834485,0.0001907367],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09662837,"threshold_uncertainty_score":0.9998195,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02368605144377168,"score_gpt":0.34229820048589,"score_spread":0.3186121490421184,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}