{"id":"W2766433963","doi":"10.3791/56281","title":"High Resolution Fluorescent &lt;em&gt;In Situ&lt;/em&gt; Hybridization in &lt;em&gt;Drosophila&lt;/em&gt; Embryos and Tissues Using Tyramide Signal Amplification","year":2017,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Developmental Biology and Gene Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"In situ hybridization; Biology; Molecular biology; Complementary DNA; Subcellular localization; Streptavidin; RNA; Horseradish peroxidase; Oligonucleotide; Messenger RNA; Cell biology; DNA; Biotin; Cytoplasm; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001085663,0.001354516,0.0006498382,0.001328253,0.001055865,0.001058003,0.001322631,0.001112947,0.01778737],"category_scores_gemma":[0.0005875298,0.00101985,0.0007542795,0.000872498,0.0009030569,0.001057998,0.0008968919,0.002206603,0.01082248],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008397679,"about_ca_system_score_gemma":0.0006509735,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001848278,"about_ca_topic_score_gemma":0.005168099,"domain_scores_codex":[0.9994327,0.00007832556,0.00005895005,0.0001952367,0.0001514965,0.00008311807],"domain_scores_gemma":[0.99955,0.00009925452,0.00006316901,0.0001270255,0.0001105647,0.00004996641],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003764162,0.00001398517,0.00008838442,0.0001138109,0.000009005887,0.0001050831,0.00003823954,0.0001209504,0.9912229,0.000861236,0.0007005055,0.006688268],"study_design_scores_gemma":[0.00003438233,0.00008490723,0.002344722,0.00004583036,0.00004180889,0.001147327,0.00004998956,0.002172596,0.9418403,0.0004414992,0.05177015,0.00002665716],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1040271,0.002288171,0.8519849,0.0007833174,0.0003148256,0.0007772646,0.005110078,0.005315582,0.02939873],"genre_scores_gemma":[0.1237994,0.004224424,0.808935,0.0005070039,0.0001201142,0.001476011,0.008453421,0.004374248,0.04811037],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01778737,"threshold_uncertainty_score":0.05950469,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02368605144377168,"score_gpt":0.34229820048589,"score_spread":0.3186121490421184,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}