{"id":"W2767150810","doi":"10.21769/bioprotoc.2584","title":"NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells","year":2017,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Cancer Research","funders":"Center for Virus Research, University of California, Irvine; Chao Family Comprehensive Cancer Center; Cancer Research Institute","keywords":"Fractionation; Cell fractionation; Nucleic acid; Compartment (ship); Cytoplasm; Extraction (chemistry); Chemistry; Cell; Chemical fractionation; Biochemistry; Chromatography; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001388391,0.001661889,0.001500181,0.001982857,0.001852177,0.001185546,0.002300463,0.001157299,0.005661341],"category_scores_gemma":[0.000917793,0.0008316752,0.001129757,0.001789358,0.001017632,0.0007207735,0.001334093,0.003866308,0.01393411],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007621824,"about_ca_system_score_gemma":0.001119485,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00153307,"about_ca_topic_score_gemma":0.003246597,"domain_scores_codex":[0.9976291,0.0003094995,0.0003272204,0.0006573102,0.0007984621,0.0002784538],"domain_scores_gemma":[0.9993548,0.0001187404,0.0000727005,0.0002317446,0.0001544853,0.00006752833],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00007863248,0.00008177366,0.00007211416,0.0003302369,0.00001182646,0.0001241798,0.000114626,0.000112775,0.9904935,0.0009112872,0.001137788,0.006531202],"study_design_scores_gemma":[0.00004662098,0.0002699702,0.001561668,0.0001046897,0.00005499332,0.0005744632,0.00005232148,0.0009508118,0.8805829,0.0008725057,0.1148721,0.00005696324],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.0854544,0.009680939,0.8251962,0.000697814,0.001849258,0.01304731,0.01843641,0.008104513,0.03753314],"genre_scores_gemma":[0.09208728,0.009711397,0.7689528,0.0009215406,0.0004084531,0.02030975,0.05124418,0.002161992,0.05420253],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005661341,"threshold_uncertainty_score":0.01893908,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02875428975749863,"score_gpt":0.369856800848796,"score_spread":0.3411025110912974,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}