{"id":"W2767150810","doi":"10.21769/bioprotoc.2584","title":"NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells","year":2017,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Cancer Research","funders":"Center for Virus Research, University of California, Irvine; Chao Family Comprehensive Cancer Center; Cancer Research Institute","keywords":"Fractionation; Cell fractionation; Nucleic acid; Compartment (ship); Cytoplasm; Extraction (chemistry); Chemistry; Cell; Chemical fractionation; Biochemistry; Chromatography; Membrane","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003138945,0.000124133,0.00008890612,0.00007064852,0.0002232332,0.0001126353,0.0002081074,0.000178807,0.0001548259],"category_scores_gemma":[0.00005178987,0.0001231555,0.00003761472,0.00003648399,0.00009133088,0.00001925738,0.00008693019,0.0001249267,0.00005299342],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003251242,"about_ca_system_score_gemma":0.00006138466,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007682122,"about_ca_topic_score_gemma":0.0004186971,"domain_scores_codex":[0.9990628,0.00007308314,0.0001625831,0.0003308012,0.0001613793,0.0002093414],"domain_scores_gemma":[0.999241,0.000005685022,0.0001283366,0.0004915553,0.00006116348,0.00007227877],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001679468,0.00007399379,0.002357675,0.00002818339,0.000008235349,0.000004224839,0.00001017494,0.000004161792,0.9731765,0.000008073564,0.001067675,0.02158163],"study_design_scores_gemma":[0.001478971,0.0006798762,0.02185217,0.000009003085,0.000001680949,0.000005268453,0.00001247251,0.00005496693,0.8349208,0.00007121299,0.1407774,0.0001361714],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.683696,0.00001533335,0.0001144586,0.0007674607,0.0001311278,0.3120211,0.00001752828,0.0000233361,0.003213643],"genre_scores_gemma":[0.5424127,0.000007347507,0.0002540018,0.0001248604,0.0002914727,0.4559771,0.00001756429,0.0000287573,0.0008862831],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1439559,"threshold_uncertainty_score":0.5022135,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02875428975749863,"score_gpt":0.369856800848796,"score_spread":0.3411025110912974,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}