{"id":"W2768505958","doi":"10.1007/s00253-017-8639-0","title":"KfoA, the UDP-glucose-4-epimerase of Escherichia coli strain O5:K4:H4, shows preference for acetylated substrates","year":2017,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"National Natural Science Foundation of China; Kidney Foundation of Canada","keywords":"Escherichia coli; Biochemistry; Chondroitin sulfate; Acetylation; Chemistry; Biosynthesis; Recombinant DNA; Uridine diphosphate; Enzyme; Glucuronic acid; Uridine diphosphate glucose; Chondroitin; Gene cluster; Glycosyltransferase; Disaccharide; Uridine; Stereochemistry; Polysaccharide; Gene; Glycosaminoglycan; RNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003513533,0.0002215034,0.000284496,0.00006219278,0.0004695784,0.00003200275,0.0006893278,0.0009515007,0.00003458151],"category_scores_gemma":[0.0001739023,0.0001621851,0.00007137839,0.00006499197,0.001501843,0.000004646518,0.0003303374,0.0002957626,0.00000987621],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007425678,"about_ca_system_score_gemma":0.00009140572,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003341832,"about_ca_topic_score_gemma":0.0002649489,"domain_scores_codex":[0.9987165,0.00004768661,0.0002802783,0.0005050851,0.00003626284,0.0004141809],"domain_scores_gemma":[0.9987344,0.00004484181,0.0003031171,0.0007720094,0.00009593065,0.00004972434],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003408268,0.00005772775,0.0002217558,0.0000282048,0.0001009274,8.672565e-7,0.00002057593,0.000002147286,0.9852398,0.004949413,0.0004638944,0.008573846],"study_design_scores_gemma":[0.001105434,0.000388734,0.001122252,0.00000679847,0.0000215894,0.00001842593,0.0001079923,0.00002198121,0.965568,0.0006157732,0.03083979,0.0001832152],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.994598,0.0007635352,0.0007282495,0.002200852,0.00007252696,0.0009284584,0.0002926057,0.00002717472,0.0003886045],"genre_scores_gemma":[0.99745,0.000461706,0.00104902,0.0002165222,0.00004698437,0.0001364343,0.0002736532,0.0000178949,0.0003478083],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0303759,"threshold_uncertainty_score":0.7338845,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02180509819300923,"score_gpt":0.2624651073881155,"score_spread":0.2406600091951062,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}