{"id":"W2768505958","doi":"10.1007/s00253-017-8639-0","title":"KfoA, the UDP-glucose-4-epimerase of Escherichia coli strain O5:K4:H4, shows preference for acetylated substrates","year":2017,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"National Natural Science Foundation of China; Kidney Foundation of Canada","keywords":"Escherichia coli; Biochemistry; Chondroitin sulfate; Acetylation; Chemistry; Biosynthesis; Recombinant DNA; Uridine diphosphate; Enzyme; Glucuronic acid; Uridine diphosphate glucose; Chondroitin; Gene cluster; Glycosyltransferase; Disaccharide; Uridine; Stereochemistry; Polysaccharide; Gene; Glycosaminoglycan; RNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000106654,0.0005676697,0.0004135717,0.0002890299,0.0001339387,0.0004544777,0.0002379048,0.0002260118,0.0008275842],"category_scores_gemma":[0.0002258238,0.0001590068,0.0002198622,0.0003238209,0.0003356721,0.000271853,0.0002054356,0.0003072889,0.0006890653],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002283908,"about_ca_system_score_gemma":0.0002263144,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002721176,"about_ca_topic_score_gemma":0.002203891,"domain_scores_codex":[0.9998277,0.0000214627,0.00001475536,0.00003808194,0.00004171194,0.00005612891],"domain_scores_gemma":[0.9997854,0.000037872,0.00003764109,0.00003009777,0.00004432392,0.00006469026],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002243801,0.00001636154,0.001172325,0.00002369575,0.000004343521,0.00005257217,0.00001218463,0.0000191886,0.9963307,0.00003167411,0.00003847223,0.002074054],"study_design_scores_gemma":[0.000004796466,0.0001261026,0.01112415,0.000003133037,0.000009769219,0.0005220521,0.00008100314,0.0002493474,0.9868276,0.00002777974,0.001016905,0.000007327777],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9956988,0.0006845539,0.001925435,0.00006696296,0.00002878736,0.000008595708,0.0004476605,0.00006779269,0.00107134],"genre_scores_gemma":[0.9945728,0.0002924257,0.001995777,0.00003790137,0.000008104334,0.00000410835,0.001466298,0.00003855555,0.001584119],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002721176,"threshold_uncertainty_score":0.005410731,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02180509819300923,"score_gpt":0.2624651073881155,"score_spread":0.2406600091951062,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}